猪生殖与呼吸综合征病毒GP5基因对小鼠的DNA免疫  

Immunization of mice with recombinant DNA vaccines encoding PRRSV GP5 gene

作　　者：任慧英[1] 杨汉春[1] 郭鑫[1] 陈艳红[1] 查振林[1] 

机构地区：[1]中国农业大学农业部预防兽医学重点实验室,北京100094

出　　处：《中国兽医科学》2006年第2期107-111,共5页Chinese Veterinary Science

基　　金：国家高技术研究发展计划(863)项目(2002AA241341);国家"十五"科技攻关计划项目(2002BA514A-16-05)

摘　　要：将表达完整猪生殖与呼吸综合征病毒(PRRSV)GP5和融合表达泛素与GP5成熟肽的真核重组表达质粒(pCI-GP5和pcDNA-U-GP5)分别肌肉注射免疫BALB/c小鼠,共免疫3次。采用间接ELISA法检测血清中抗GP5的抗体水平,MTT法检测脾淋巴细胞的增殖能力,LDH释放试验检测脾NK细胞的杀伤活性,并通过流式细胞术检测脾淋巴细胞亚群的比例。结果发现,2种DNA重组质粒免疫后均可诱导小鼠产生体液免疫和细胞免疫。三免后第5周,pCI-GP5质粒免疫组诱导产生的抗GP5抗体水平及NK活性明显高于pcDNA-U-GP5质粒免疫组;而pcDNA-U-GP5免疫组的淋巴细胞增殖指数及CD4-CD8+T细胞比例明显高于pCI-GP5组。表明,GP5基因可诱导产生较高的免疫反应,泛素与GP5的融合表达可增强PRRSV GP5诱导的细胞免疫反应。BALB/c mice were vaccinated intramuscularly with DNA vaccine vectors pCI-GP5 and pcDNA-U-GP5 which contain porcine reproductive and respiratory syndrome virus （PRRSV） GP5 gene with or without porcine ubiquitin gene. On week 5 after the third immunization, mice in pCI-GP5 group showed much higher antibody levels and percentage of NK lysis than that in pcDNA-U-GP5 group. A significantly enhanced stimulation index and a significantly increased percentage of CD4^- CD8^＋ T cells were observed in pcDNA-U-GP5 group compared with that in pCI-GP5 group. The results demonstrated that DNA immunization against PRRSV induced the production of both humoral-and cell-mediated immune responses in mice. The pcDNA-U-GP5 could elicit much stronger cellular immune response.

关 键 词：猪生殖与呼吸综合征病毒 GP5 泛素 DNA免疫 小鼠 

分 类 号：S852.659.6[农业科学—基础兽医学] S852.4[农业科学—兽医学]