芪芍五味子复方制剂抗氧自由基作用的实验研究  

The anti-oxygen-free-radical effect of compound traditional Chinese medicine, including radix astragali, radix paeoniae rubra, fructus schisandrae on viral myocarditis model mice

作　　者：刘奉琴[1] 王玉林[1] 靳有鹏[1] 王青青[1] 

机构地区：[1]山东大学山东省立医院儿科,山东济南250021

出　　处：《山东大学学报（医学版）》2006年第2期188-192,共5页Journal of Shandong University:Health Sciences

基　　金：山东省自然科学基金资助项目(Y2003C02)

摘　　要：目的:研究中药芪芍五味子复方制剂对柯萨奇B3(CVB3)病毒性心肌炎(viralmyocarditis,VM)小鼠心肌的保护作用。方法:BALB/c小鼠随机分为正常对照组、病毒对照组、中药高剂量组、中药中剂量组、中药低剂量组及维生素C和病毒唑联用组。各组于CVB3感染后第3、7、14、21天分批处死,取心脏作组织病理学检查,同时分别用黄嘌呤氧化酶法和硫巴比妥酸法测血清超氧化物歧化酶(SOD)活性及丙二醛(MDA)含量。结果:3个中药组在第14、21天血清SOD活性均明显高于病毒对照组,MDA含量明显低于病毒对照组(P﹤0.05);高剂量组、中剂量组在第21天血清SOD活性和MDA含量基本恢复正常;同时各中药组小鼠心肌炎症性病理变化均明显减轻。结论:芪芍五味子复方制剂能减轻CVB3感染小鼠氧自由基损伤,对VM小鼠心肌具有保护作用。Objective： To study the of compound traditional Chinese medicine, anti-oxygen-free-radical effect and myocardial protective effect including Radix Astragali, Radix Paeoniae Rubra, Fructus Schisandrae on viral myocarditis （VM） model mice. Method： Two hundred and ninty male BALB/c mice in- oculated with CoxsackievirusB3, were randomly divided into normal control group （A）, VM control group（B）, high dose（C）, medium dose（D）, low dose（E）treatment group, and V iaminC plus ribavirin treatment group（F）. Mice were sacrificed on the 3, 7, 14, 21th day. The symptoms, mortality, pathologic changes of myocardium were observed. Serum value of SOD was detected by Xanthine Oxydase method, and the content of MDA by TBA （thibabituric acid） method. Results： The mortality and myocardial pathologic scores reduced in groups C,D,E with dose-dependence. The serum value of SOD was higher and the content of MDA was much lower in compound medicine treated groups. Moreover, the serum SOD and MDA got normal in groups C and D onthe 21th day. Conclusion： This Chinese compound medicine meliorates free radical injure and protects myocardium in VM mice.

关 键 词：芪芍五味子 心肌疾病 柯萨奇病毒感染 氧自由基 小鼠 近交BALB C 

分 类 号：R286[医药卫生—中药学]