氯血红素诱导人脐静脉内皮细胞血红素氧合酶表达及其对血管保护作用  

Effects of hemin on the expression of hemeoxygenase in human umbilical vein endothelial cells

作　　者：郭钰珍[1] 陈汉平[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院妇产科,湖北武汉430030

出　　处：《中国医师杂志》2006年第2期173-175,共3页Journal of Chinese Physician

摘　　要：目的观察氯血红素对血管内皮细胞血红素氧合酶(HO)表达和内源性一氧化碳(CO)的影响。方法体外培养人脐静脉内皮细胞,用氯血红素和原卟啉锌,以诱导和阻断HO-1的表达。通过逆转录聚合酶链式反应和免疫细胞化学法检测HO-1、HO-2 mRNA和蛋白的表达,酶联免疫法检测培养上清液中碳氧血红蛋白(COHb)含量,四唑氮比色法检测细胞增殖。结果HO蛋白主要定位于在内皮细胞胞浆,HO-2的表达高于HO-1;氯血红素显著增加血管内皮细胞HO-1 mRNA、蛋白质的表达及培养上清液中COHb含量,同时促进细胞增殖。结论HO-1表达和内源性CO增加是保护内皮细胞的分子生物学基础,提示HO/CO信号系统在心血管病的发生和发展中具有重要作用。Objective To observe the effects of hemin on the expression of hemeoxygenase （HO） on the human umbilical vein endothelial cells. Methods The hemin and zinc protoporphyrin-Ⅸ were added in the cultured endothelial cells to induce and inhibit the ex pression of HO-1, respectively. The expressions of HO-1 and HO-2 mRNA were detected using reverse transcription polymerase chain （ RT- PCR） and the relative amount of CO released into the media was quantitated as carboxyhemoglobin （COHb） by enzyme linked immunosorbent assay （ELISA）. The proliferation of endothelial cells was detected by methyl thiazolyl tetrazolium test （MTT）. Results The expression of HO protein was mainly detected in the cytoplasm of endothelial cell. The expression of HO-1 mRNA in endothelial cell and the amount of COHb in the media increased significantly with the treatment of hemin. Conclusion The expression of HO-1 and the increase of endogenous CO are the molecular bases of the proliferation of endothelial cells, and the HO/CO system may play an important role in the development of cardiovascular diseases.

关 键 词：氯化血红素 脐静脉 内皮细胞 血红素氧化酶(脱环) 

分 类 号：R54[医药卫生—心血管疾病]