化学致癌物转化细胞端锚聚合酶mRNA的表达  

Expression of Tankyrase mRNA in Three Chemical Carcinogens-induced Malignant Transformed Cells

作　　者：王敏[1] 吕嘉春[1] 吴中亮[1] 陈家堃[1] 

机构地区：[1]广州医学院化学致癌研究所,广东广州510182

出　　处：《环境与职业医学》2006年第1期52-53,共2页Journal of Environmental and Occupational Medicine

基　　金：国家自然科学基金项目(编号:30200235)

摘　　要：[目的]探讨3种化学致癌物诱发细胞恶变后tankyrasemRNA的表达。[方法]用反转录-聚合酶链反应(RT-PCR)技术检测正常细胞和恶性转化细胞tankyrasemRNA的表达。[结果]tankyrasemRNA在转化细胞中的表达高于正常细胞。[结论]3种化合物诱发的细胞恶性转化涉及了tankyrase活性改变,提示tankyrasemRNA高表达可能与化学致癌有关。[ Objective ]-To study the expression of tankyrase in malignant transformed human bronchial epithelial cells induced by three chemical carcinogens. [ Methods ] Reverse transcription-polymerase chain reaction （ RT-PCR ）was used to measure the expression of tankyrase mRNA in 16HBE and malignant transformed cells. [ Results ] The expression of tankyrase in malignant transformed cells was higher than that in normal cells.[Conclusion ] The overexpression of tankyrase in the transformed cells might be assoctiated to the chemical carcinogenesis.

关 键 词：化学致癌物 人支气管上皮细胞 恶性转化细胞 聚合酶 

分 类 号：R994.3[医药卫生—毒理学]