全脑缺血模型中不同缺血时间参数对预处理保护效应的影响  

THE EFFECT OF TIME PARAMETERS OF CEREBRAL ISCHEMIC PRECONDITIONING ON ITS PROTECTIVE EFFECT AGAINST GLOBAL CEREBRAL ISCHEMIC INJURY IN RATS

作　　者：刘惠卿[1] 李文斌[2] 冯荣芳[1] 李清君[2] 周爱民[2] 赵宏岗[2] 羡晓辉[2] 艾洁[2] 

机构地区：[1]河北省人民医院神经内科,河北石家庄050051 [2]河北医科大学基础医学研究所病理生理学研究室,河北石家庄050017

出　　处：《中国应用生理学杂志》2006年第1期7-11,i0002,共6页Chinese Journal of Applied Physiology

基　　金：河北省自然科学基金资助项目(302494)

摘　　要：目的：观察脑缺血预处理（CIP）的持续时间、CIP与后续损伤性缺血之间的间隔时间对CIP抗全脑缺血所致海马锥体神经元迟发性死亡（DND）作用的影响。方法：采用四血管闭塞法（4VO），制作大鼠全脑缺血模型。脑组织切片硫堇染色法观察海马CA1区锥体神经元DND程度，确定组织学分级（HG）。结果：Sham组和3minCIP组海马未见DND。损伤性脑缺血组海马CA1区有明显的DND，其中6min、10min缺血组的HG为2～3级，15min缺血组的HG主要为3级。CIP＋损伤性脑缺血组中，3min-3d-6min（3minCIP后间隔三天给予6min损伤性脑缺血，下同）和3min-3d-10min组DND不明显，提示CIP可有效地保护海马CAl区神经元，防止6min或10min损伤性脑缺血诱导的DND。在3min-1d-10min组和3min-3d-15min组中，CIP的保护效应较3min-3d-10min组明显减弱。定量分析CIP对海马神经元的保护效应发现，3min-3d-6min组的神经元保护数（PN）和保护指数（PI）与3min-3d-10min组相比无明显差别（P〉0．05）；但3min-3d-10min组的神经元增长指数（GI）较3min一3d一6min组明显升高（P〈0．05）。结论：虽然3min-3d-6min组与3min-3d-10min组中CIP对神经元的保护作用相近，但3min-3d-10min组中，CIP的保护作用更容易被观察到，且CIP的保护潜能可得到最大程度的显现。应用3min-3d-10min组的时间参数建立全脑缺血耐受模型可以诱导出CIP最大的保护潜能。To investigate the effects of the duration of cerebral ischemic preconditioning（CIP） and interval between CIP and the subsequent ischemic insult on the protection of CIP against delayed neuronal death（DND） in the CA1 hippocampus normally induced by brain ischemic insult. Methods： Four-vessel occlusion cerebral ischemic model of rats（54 ） was used. The brain of the rats was sectioned and stained with thionin to show DND in the CA1 hippocampus. Results： No DND was found in the hippoeampus of the rats subjected to sham operation and CIP, in which 3 min cerebral ischemic preconditioning was performed. Obvious destruction of the CA1 hippocampus was found in brain ischemic insult group, in which histological（HG） was 2--3 in 6 min and 10 min ischemia subgroups and grade 3 in 15 min ischemia subgroup. In CIP ＋ brain ischemic insult group, no obvious neuronal damage was found in 3min-3d-6min（CIP for 3 min was followed by a brain ischemic insult for 6 min at an interval of 3 d, the same as the following） and 3 min-3 d-10 min groups, indicating that CIP effectively protected neurons of the CA1 hippoeampus against DND normally induced by ischemic insult for 6 or 10 min. However, in 3 min-1 d-10 min and 3 min-3 d-15 min groups, the protective effect of CIP was lower than that in the 3 min-3 d-10 min group. The quantitative analysis of the protective effect of CIP on the CA1 hippocampal neurons showed that there was no significant difference in protecting number and protecting index between 3 min-3 d-6 min and 3 min-3 d-10 min groups （P〉0.05）. However, the growth index in 3 min-3 d-10 min group was obvious larger than that in 3 min-3 d-6 min （P 〈 0.05）. Conclusion： Although the protective effects of CIP in 3 min-3 d-6 min and 3 min-3 d-10 min groups were similar, the protective effect of CIP in 3 min-3 d-10 min group was sensitively found. Maximal protective potential of CIP could be induced when using the time parameters of 3 min-3 d-10 min to establish the model of global cerebral isch

关 键 词：脑缺血预处理 时间参数 海马 大鼠 

分 类 号：Q426[生物学—神经生物学] R338[生物学—生理学]