L-精氨酸和氨基胍对大鼠内毒素性肺损伤治疗作用的实验研究  

EFFECTS OF L-ARGININE AND AMINOGUANIDINE ON THE ENDOTOXIN INDUCED LUNG INJURY IN RATS

作　　者：张建新[1] 李立萍[1] 董淑婷[2] 李兰芳[1] 解丽君[1] 梁良[1] 

机构地区：[1]河北省医学科学院,河北石家庄050021 [2]石家庄市第三医院,河北石家庄050011

出　　处：《中国应用生理学杂志》2006年第1期85-89,共5页Chinese Journal of Applied Physiology

基　　金：国家人事部留学人员重点资助项日(9900789);河北省博士基金资助项目(99547015D)

摘　　要：目的:观察L-精氨酸(L-arginine)和一氧化氮合酶抑制剂氨基胍(AG)对内毒素性肺损伤的治疗作用。方法:采用静脉注射脂多糖(LPS)制备内毒素性肺损伤大鼠模型。将48只SD大鼠随机分为6组:空白对照组、LPS模型组、AG治疗组(50mg/kg)、L-精氨酸(500mg/kg)、(250mg/kg)和L-精氨酸(250mg/kg)+AG(50mg/kg)治疗组。经腹腔给药,实验过程中监测大鼠平均动脉压(MAP),定时取静脉血测定血浆中NO含量,于规定时间处死大鼠,迅速取出肺脏,观察LPS引起大鼠急性肺损伤后肺系数、肺水肿情况和肺组织中丙二醛(MDA)含量、一氧化氮合酶(NOS)、超氧化物歧化酶(SOD)活性的变化,以及L-精氨酸和氨基胍分别单独给药和二者联合给药对内毒素性肺损伤的治疗作用。结果:氨基胍可明显升高MAP,降低肺系数和肺含水量,减少血浆中NO含量,可显著降低肺组织中NOS活性,减少MDA含量,增强SOD活性,改善肺损伤;L-精氨酸可明显降低肺系数和肺含水量,减少MDA含量,增强SOD活性;L-精氨酸与氨基胍联合应用亦得到上述类似结果。结论:L-精氨酸和氨基胍分别单独给药以及二者联合给药对内毒素性肺损伤均具有治疗作用。Aim： To investigate the effects of L-arginine and nitric oxide synthase（NOS） inhibitor Aminoguanidine（AG） on endotoxin induced lung injury in rats. Methods： Forty eight healthy male SD rats weighing（300±20）g were used. The animals were anesthetized with 20 % urethane 1 g·kg^-1. Common carotid artery（CAA） and common carotid vein （CAV） were exposed through a median incision in the neck. Mean arterial pressure（MAP） was measured through a pressure transducer connected with intubation of CAA. The animals were randomly divided into six groups： group 1 ： control ： group 2 ： LPS （5 mg·kg^-1 intravenous injection, iv） ; group 3 ： AG （50 mg·kg^-1 intraperitoneal injection, IP） ; group 4： high dose L-arginine（500 mg·kg^-1 IP） ; group 5. low dose L-arginine （250 mg＇kg-t IP）. Group 6： L-arginine ＋ AG （250 mg·kg^-1, 50 mg·kg^-1 IP）. Group 1： The animals were killed 6 h after 0.9% saline solution was given. Group 2 ： 0.9 % saline solution was given 3 h after LPS iv and the animals were killed 3 h after medication. Group 3,4, 5 and 6： AG, L-arginine and L-arginine＋ AG were given 3 h after LPS iv respectively and the animals were killed 3 h after medication respectively. The pulmonary was removed immediately. The pulmonary coefficient and water content in pulmonary tissue were calculated（% ）. The NO content in plasma, MDA content and NOS, SOD activity in the pulmonary tissue were measured. Results： L-arginine, AG and L-arginine ＋ AG significantly decreased pulmonary coefficient and water content in pulmonary tissue and ameliorated endotoxin induced lung injury. AG and L-arginine ＋ AG significantly decreased NO content in plasm, decreased MDA content and inhibited NOS activity and enhanced SOD activity in the pulmonary tissue. Conclusion： It may be concluded that L-arginine, AG and L-arginine ＋ AG have beneficial effects on lung injury induced by LPS.

关 键 词：肺损伤 一氧化氮 L-精氨酸 氨基胍 脂多糖 

分 类 号：R332[医药卫生—人体生理学]