血管钠肽对大鼠肠系膜动脉平滑肌细胞Ca^(2+)激活K^+通道的作用  

EFFECT OF VASONATRIN PEPTIDE ON THE Ca^(2+) ACTIVATED K^+ CHANNELS OF VASCULAR SMOOTH MUSCLE CELLS ISOLATED FROM RAT MESENTERY ARTERIES

作　　者：于军[1] 朱妙章[2] 刘利兵[1] 陈宝莹[3] 吕顺艳[2] 周京军[2] 付兆君[4] 

机构地区：[1]第四军医大学教学实验中心,陕西西安710032 [2]第四军医大学生理学教研室,陕西西安710032 [3]第四军医大学唐都医院放射科,陕西西安710032 [4]第四军医大学航空航天生理学教研室,陕西西安710032

出　　处：《中国应用生理学杂志》2006年第1期94-97,共4页Chinese Journal of Applied Physiology

基　　金：国家自然科学基金资助项目(39970327)

摘　　要：目的:研究血管钠肽(VNP)对大鼠肠系膜动脉血管平滑肌细胞(VSMCs)Ca2+激活K+通道(KCa)的作用及其机制。方法:采用全细胞膜片钳技术观察VNP对KCa的影响,以及HS-142-1、8-Br-cGMP和美蓝(MB)在这一过程中的作用。结果:①VNP(10-6mol/L)显著增强KCa(P<0.05,n=5)。②8-Br-CGMP(10-3mol/L)模拟VNP增强KCa的作用(P<0.05,n=6)。③HS-142-1(2×10-5mol/L)或MB(10-5mol/L)完全阻断VNP增加KCa电流密度的作用。结论:VNP通过作用于VSMCs的钠尿肽GC耦联受体,升高细胞内的cGMP水平,激活KCa。Aim： To investigate effect and mechanism of vasonatrin peptide（VNP） on Ca^2＋ activated K^＋ channels（KCa） of vascular smooth muscle cells（VSMCs） isolated from rat mesentery arteries. Methods： Changes of Kc, induced by VNP were measured by the means of whole cell recording mode of patch clamp, furthermore effects of HS-142-1 （0.3 g/L）, 8-Br-cGMP and methylene blue（MB） were observed. Results： KCa was significantly enhanced by VNP（ 10^-6 mol/L）, which was mimicked by 8-Br-cGMP（ 10^-3 mol/L） and blocked completely by HS-142-1 or MB（2 ×10^-5 mol/L）. Conclusion： VNP increases KCa of VSMCs isolated from rat mesenteric arteries, by binding with natriuretic peptide guanylate cyclase-coupled receptors and increasing the intracellular level of cGMP in VSM- Cs.

关 键 词：血管钠肽 肠系膜动脉 Ca^2+激活K^+通道 血管平滑肌细胞 

分 类 号：R33[医药卫生—人体生理学]