原发性肝癌患者红细胞调控IL-8新的实验体系研究  

Studies on a new experimental system of erythrocyte regulating IL-8 in patients with primary hepatocarcinoma

作　　者：张乐之[1] 郭峰[2] 许育[1] 吴淑梅[1] 王海立[1] 

机构地区：[1]长海医院实验诊断科,上海200433 [2]长海医院输血科,上海200433

出　　处：《解放军医学杂志》2006年第2期98-99,共2页Medical Journal of Chinese People's Liberation Army

摘　　要：目的采用血液免疫反应路线图实验体系探讨原发性肝癌患者红细胞调控白细胞分泌IL-8的能力。方法将癌细胞(S180)悬液0·2ml(5×106/ml)或生理盐水0·2ml加至枸橼酸抗凝全血细胞悬液(或白细胞悬液)0·2ml和血浆0·3ml中,37℃水浴1h,酶联免疫法测定反应液中IL-8含量。结果原发性肝癌患者全血细胞实验组、全血细胞对照组、白细胞实验组和白细胞对照组的IL-8含量(pg/ml)分别为376·35±243·96、353·64±271·92、461·27±277·11和424·97±278·93,正常人相应各组则分别为11·36±6·93、4·98±4·35、29·41±30·66和20·77±24·20;原发性肝癌患者全血细胞实验组、白细胞实验组和正常人全血细胞实验组、白细胞实验组的癌细胞激活率(%)分别为0·22±0·24、0·25±0·53、2·49±2·33和0·75±0·21;原发性肝癌患者全血细胞实验组、全血细胞对照组和正常人全血细胞实验组、全血细胞对照组的红细胞IL-8吸附率(%)分别为0·22±0·18、0·17±0·33、1·18±2·29和0·86±0·49。原发性肝癌患者红细胞IL-8激活率及IL-8吸附率均明显低于正常人(P<0·01)。结论采用血液免疫反应路线图实验体系测定红细胞对IL-8的调控作用方法简便、实用。原发性肝癌患者红细胞对IL-8含量的调控能力明显下降。Objective To approach the capacity of erythrocyte regulating IL-8 in patients with primary hepatocarcinoma （PHC） by a new experimental system of hemaimmune Methods 0. 2ml suspension of cancer cells （S180： 5× 10^6/ml） or NS were added into 0. 2ml anticoagulant suspension of whole blood cells or leukocytes and 0. 3ml plasma, then incubated for 1 h at 37℃. The content of IL-8 was determined by ELISA. Results In the patients with PHC, the IL-8 levels （pg/ml） in experimental and control groups of whole blood cells, and in experimental and control groups of leukocytes were 376. 35 ± 243. 96, 353. 64 ± 271.92, 461.27 ± 277.11 and 424. 97 ± 278. 93, respectively; while in the normal human, they were 11. 36±6. 93, 4. 98±4. 35, 29. 41±30. 66 and 20. 77±24. 20, respectively. In the patients with PHC, the activation rates of cancer cells in the experimental groups of both whole blood cell and leukocyte were 0. 22 ±0. 24 and 0. 25±0. 53, respectively; while in normal human, they were 2. 49±2. 33 and 0. 75±0. 21, respectively. In the patients with PHC, the IL-8 adsorption rate of erythrocyte in both experimental and control groups of whole blood cell were 0. 22±0. 18 and 0. 17± 0. 33, respectively; while in normal human, they were 1. 18±2. 29 and 0. 86±0. 49, respectively. The IL-8 activated rates of erythrocyte in the patients with PHC was much lower than in the normal human （P〈0. 01）, and so was the adsorption rate of erythrocyte （P〈 0. 01）. Conclusion The results indicated that the experimental system of hemaimmune reaction, when used in detecting the regulative effect of erythrocyte to IL-8, was practical and convenient. The regulative capacity of erythrocyte to IL-8 declined obviously in the patients with PHC.

关 键 词：癌 肝细胞 变态反应和免疫学 红细胞 白细胞介素8 

分 类 号：R392[医药卫生—免疫学]