小麦体细胞杂种山融3号耐盐相关SSR标记的筛选和初步定位  

Screening and Localization of SSR Markers Related to Salt Tolerance of Somatic Hybrid Wheat Shanrong No.3

作　　者：单雷[1,2] 赵双宜[1] 陈芳[1] 夏光敏[1] 

机构地区：[1]山东大学生命科学学院 [2]山东省农业科学院高新技术研究中心,济南250100

出　　处：《中国农业科学》2006年第2期225-230,共6页Scientia Agricultura Sinica

基　　金：国家自然科学基金重点项目(30530480);国家"973"项目(2006CB100106);国家"863"课题(2005AA241051)资助

摘　　要：【目的】寻找并定位与小麦耐盐性有关的SSR标记。【方法】选取耐盐性强的体细胞杂交新品种山融3号为试验材料。以山融3号为母本,盐敏感的常规品种济南17为父本,配制杂交组合(01组合)。利用SSR-BSA(bulkedsegregantanalysis)方法对01组合F2代分离群体苗期耐盐性进行鉴定,并结合小麦SSR图谱分析,标记其耐盐相关位点。【结果】通过遗传分析和χ2检验表明:01组合中的耐盐性状可能由一个主效基因控制。应用SSR标记技术,筛选到了与山融3号耐盐性状连锁的SSR标记Xgwm304。【结论】SSR标记Xgwm304位点与主效耐盐基因间的遗传距离为24.41cM,该主效耐盐基因定位于5A染色体的短臂上。[Objective] Screen and localize the SSR markers related to salt tolerance trait in wheat variety Shanrong No.3. [Method] Using a novel salt-tolerant wheat asymmetric somatic hybrid variety Shanrong No.3 as experimental material, the F2 population of hybridized combination 01 was constructed from sexual crossing between Shanrong No.3 and salt-sensitive variety Jinan 17. In combination to the SSR map of wheat, the seedlings of the population were used to determine the salt tolerance and the loci related to the salt tolerance was tagged by microsatellite （SSR） and BSA （bulked segregant analysis）. [Result] Genetic analysis and chi-square test of salt-tolerant phenotype of F2 population showed that the salt tolerance of the combination 01 was likely controled by major gene. A wheat specific SSR marker Xgwm304 was decided to link with salt-tolerant locus of wheat variety Shanrong No.3. [ Conclusion ] The genetic distance between Xgwm304 and the critical gene is 24.41 cM （centMorgan）. The major salt tolerance gene in wheat variety Shanrong No.3 has been located on 5AS of wheat chromosome.

关 键 词：小麦体细胞杂种 耐盐基因 SSR标记 染色体定位 

分 类 号：S512.1[农业科学—作物学]