河北地区花生根瘤菌的系统发育多样性研究  

Phylogenetic Diversity of Rhizobia Isolated from the Root Nodules of Peanut (Arachis hypogaea) Grown in Hebei

作　　者：刘杰[1,2] 汪玲玲[2] 汪恩涛[2,3] 李颖[2] 陈文新[2] 

机构地区：[1]青岛科技大学化工学院生物与制药工程系 [2]中国农业大学生物学院微生物系,北京100094 [3]墨西哥国立理工大学生物与技术学院微生物系,墨西哥城

出　　处：《中国农业科学》2006年第2期344-352,共9页Scientia Agricultura Sinica

基　　金：国家重点基础研究发展计划"973"项目(2001CB108905);青岛科技大学引进人才启动基金资助

摘　　要：【目的】了解中国北方地区花生根瘤菌的多样性状况。【方法】采用16SrDNAPCR-RFLP、16S～23SIGSPCR-RFLP和16SrRNA基因的序列分析方法对分离自河北地区的58株花生根瘤菌以及Rhizobium、Agrobacterium、Sinorhizobium、Mesorhizobium和Bradyrhizobium的参比菌株进行系统发育多样性比较分析。【结果】16SrDNAPCR-RFLP方法在细菌属水平上聚群良好;而16S～23SIGSPCR-RFLP则适于属和种水平上的聚群。3种方法在系统发育上得到基本一致的结果。其中有38株花生根瘤菌是与Bradyrhizobium关系密切的慢生菌株,并且与B.japonicum和B.liaoningense的关系最为接近,与有关中国西南地区和华中地区花生根瘤菌的报道结果相似。然而供试菌株中还存在6株快生和10株中慢生花生根瘤菌,其中快生菌在系统发育关系上接近R.yanglingense、R.mongolense和R.gallicum。【结论】河北地区的花生根瘤菌具有更大的多样性。[ Objective ] We hope to understand the diverse state of peanut rhizobia in northern part of China. [ Method ] Fifty eight rhizobial strains isolated from root nodules of peanut plants grown at different sites in Hebei Province were analyzed and compared with the reference strains of the species of Rhizobium, Agrobacterium, Sinorhizobium, Mesorhizobium and Bradyrhizobium by 16S rDNA PCR-RFLP, 16S-23S IGS PCR-RFLP and 16S rRNA gene sequencing. [Results] The results showed that thirty eight strains belonged Bradyrhizobium related to Bradyrhizobiumjaponicum and Bradyrhizobium liaoningense. [Conclusion] It demonstrates that peanut plants nodulate mainly with Bradyrhizobium strains in Hebei, similar to the previous reports with rhizobia from peanut plants grown in other regions of China. However, the existence of six fast-growing rhizobial strains related to R. yanglingense, R. mongolense, and R. gallicum and of ten moderately slow-growing strains indicate that peanut plants attract diverse rhizobia in Hebei Province.

关 键 词：花生根瘤菌 系统发育多样性 河北 

分 类 号：S565.2[农业科学—作物学] S154.381