罗汉果皂甙清除自由基及抗脂质过氧化作用的研究  

Study on the Inhibitory Effects of Natural Sweetner Mogrosides on Radical and Lipid Peroxidation

作　　者：戚向阳[1] 陈维军[1] 张俐勤[1,2] 单夏锋[3] 宋云飞 

机构地区：[1]华中农业大学食品科技学院 [2]浙江嘉兴职业技术学院生物工程系,嘉兴314000 [3]中国农业科学院茶叶研究所 [4]桂林莱茵生物制品有限公司

出　　处：《中国农业科学》2006年第2期382-388,共7页Scientia Agricultura Sinica

基　　金：华中农业大学创新基金

摘　　要：【目的】研究罗汉果皂甙提取物及罗汉果皂甙V的体外抗氧化活性。【方法】采用D-脱氧核糖法、抗超氧阴离子试剂盒法及比色法分析罗汉果皂甙清除自由基及抗脂质过氧化的效果。【结果】(1)罗汉果皂甙提取物(MG)均能有效地清除自由基,可抑制大鼠肝组织的脂质过氧化,对Fe2+和H2O2诱导的肝组织过氧化损伤具有保护作用,能减少红细胞溶血的发生。(2)罗汉果皂甙V清除羟基自由基的效果与MG相当,但对O2-–的清除作用优于MG。【结论】罗汉果皂甙提取物具有抗氧化活性,罗汉果皂甙V是提取物中主要的抗氧化活性成分。[Objective] The antioxidant activity of mogroside extract （MG） and mogroside V in vitro was studied. [Method] D-deoxyribose method, reagent box method and spectrophotometry method were used to determine the inhibitory effect on radical, hemolysis of red blood cells and lipid peroxidation. [ Result ] （1） It was found that mogroside extract could scavenge different radicals effectively, protect liver tissue in rat from peroxidation induced by Fe^2＋ or H2O2 and inhibit the hemolysis of red blood cells. （2） The scavenging OH effect of mogroside V was the same as mogroside extract, but its scavenging O2 effect was better than that of MCt [Conclusion] It is concluded that mogroside extract has antioxidant effects and mogroside V may be the main antioxidant components of the extract.

关 键 词：罗汉果皂甙提取物 罗汉果皂甙V 抗氧化作用 

分 类 号：S668.9[农业科学—果树学]