中华蚱蜢精子发生过程中花生凝集素受体的分布与变化  

Distribution and Changes of PNA-binding Glycoconjugates during Spermatogenesis of Acrida cinerea

作　　者：欧阳霞辉[1] 奚耕思[1] 

机构地区：[1]陕西师范大学生命科学学院

出　　处：《动物学杂志》2006年第1期7-11,共5页Chinese Journal of Zoology

基　　金：陕西省自然科学基金(No.2000SM22)

摘　　要：采用生物素标记的花生凝集素(PNA)对中华蚱蜢(Acrida cinerea)精子发生过程中细胞质膜的糖蛋白(含有糖基Gal--β(1,3)-GalNAC)进行了标记,旨在认识精子发生过程中细胞质膜糖复合物的形成与变化规律,探讨其所具有的功能。结果表明,在中华蚱蜢的精子发生中,精母细胞能够自身合成PNA受体;精子细胞到成熟精子时期生精细胞质膜糖复合物发生了明显的修饰变化,这些变化与精子获得及卵子进行识别、粘附、结合等受精能力密切相关。The distribution of PNA-binding glycoconjugates on the plasma membrane of testictilar germ cells during spermatogenesis of Acrida cinerea was detected by using biotin-labeled PNA. In testis, PNA receptors were firstly observed in the cytoplasm and on the plasma membrane of primary spermatocyte, greatly increased on the plasma membrane of secondary spermatocyte, and then decreased and accumulated in the tail of transforming spermatid. The reaction was also observed in spermatozoa and spermatheca, with stronger staining in the head and weaker staining in the tail. A universal staining pattern was observed on the plasma membrane of sperm cyst cells. The results suggest that PNA receptors are synthesized by spermatogenetic cells at early period and greatly modified during spermatogenesis, which is similar to the changes in other insects and mammals.

关 键 词：中华蚱蜢 精子发生 糖蛋白 PNA 

分 类 号：Q954.434[生物学—动物学]