Ce^(3+)对Cu^(2+)胁迫下菹草叶片Cu毒害的缓解效应研究  

Ce^(3+)-abating Effects on the Damage of Cu^(2+) Stress to Potamogeton crispus Leaves

作　　者：陈苏雅[1] 施国新[1] 丁秉中[1] 徐勤松[1] 王学[1] 

机构地区：[1]南京师范大学生命科学学院,南京210097

出　　处：《西北植物学报》2006年第2期282-289,共8页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家自然科学基金(30370083);教育部科学技术研究重点项目(01043);江苏省教育厅自然科学基金(03KJB180062);江苏省重点实验室开放基金项目资助

摘　　要：在6种不同浓度的铈(C e3+)对0.1 m g.L-1的Cu2+毒害下,研究了菹草叶片中保护酶SOD、POD、CAT的活性,活性氧H2O2,膜脂过氧化产物M DA含量及叶绿素含量等的变化及影响.结果表明,在9~12 d之内,7.5 m g.L-1以下的C e3+可以增强SOD、CAT、POD活性,降低M DA的含量,提高叶片中叶绿素和可溶性蛋白含量,从而减轻Cu2+对菹草植物体的伤害.而随着C e3+作用时间的延长和浓度的增大,C e3+的缓解作用不断减弱,C e3+和Cu2+产生协同效应,加重毒害.本实验结果认为,5~7.5 m g.L-1的C e3+缓解菹草叶片Cu2+毒害效果最好.Ce^3＋ effects on SOD,POD,CAT activities,and H2O2,MDA and chlorophyll contents of the Potamogeton crispus leaves suffering Cu^2＋ damage were studied at six Ce^3＋ concentrations. The results indicated that Ce^3＋ at the concentration of less than 7.5 mg · L ^-1 could increase SOD,POD,CAT activities,reduce MDA content,increase chlorophyll and soluble protein contents within 9-12 days,so that it could abate Cu^2＋ damage to P. crispus. As Ce^3＋ action prolonged and Ce^3＋ concentration increased,Ce^3＋-abating effect on the damage declined in intensity,and even became synergistic so as to make Cu^2＋ damage worsen. The study indicated that Ce^3＋ at the concentration of 5-7.5 mg · L ^-1 performed best in abating Cu^2＋ damage to P, crispus leaves.

关 键 词：CE^3+ CU^2+ 菹草 抗氧化酶系统 缓解效应 

分 类 号：Q945.78[生物学—植物学]