Cucumis属3种不同倍性种间杂交后代的同工酶分析  

Isoenzyme Analyses of Progenies of the Crosses of Cucumis Species with Different Ploidities

作　　者：罗向东[1] 戴亮芳[1] 钱春桃[1] 陈劲枫[1] 

机构地区：[1]南京农业大学园艺学院作物遗传与种质创新国家重点实验室,南京210095

出　　处：《西北植物学报》2006年第2期295-299,共5页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家自然科学基金项目(30470120);国家"863"计划项目(2004AA241120);江苏省高校高新技术产业发展项目(JHB04-012);PickleSeedResearchFundofPicklePackerInternational;Inc

摘　　要：以3种不同倍性的Cucum is属种间杂交后代[异源四倍体C.hy tivus(2n=4x=38,HHCC)、异源三倍体(2n=3x=26,HCC)和正反交种间杂种F1(2n=2x=19,HC/CH)]及其双亲为试材,比较研究了过氧化物酶(POD)和酯酶(EST)同工酶在不同器官中的酶谱表达特性.结果表明,花蕾中的POD和EST同工酶谱带都比叶片中的丰富,表现出明显的组织特异性.在相同器官的同一酶系统中,3种不同倍性种间杂交后代的酶谱基本一致,主要表现为互补双亲的酶带,同时出现了双亲所没有的酶带(POD4c和EST3b等),表明远缘杂交扩大了黄瓜的遗传基础.此外,在幼叶和花蕾的POD同工酶中,大部分酶带活性随染色体倍性增加而减弱,表明基因剂量与POD同工酶酶谱的表达呈负相关.The progenies of the crosses of Cucurnis species with different ploidities Eallotetraploid C. hytivus （ 2n = 3x = 26 ,HHCC） ,allotriploid （2n = 3x= 26 ,HCC） and reciprocal F1 hybirds （2n = 2x = 19,HC/CH）] and their parental materials were comparatively examined to study the zymogram expressions of POD and EST isoenzymes in different organs. The results showed that the zymograms of POD and EST isoenzymes were richer in the flower buds than in the leaves,presenting obvious tissue specificity. In the same organs, the zymograms of the progenies of the crosses of Cucurnis species with different ploidities were essentially identical ,mainly appearing as the ones of the mutually complemented parental pairs as well as presenting the ones that the parental pairs lacked ,which indicated that distant hybridization could widen the genetic base of cucumber. In addition,a majority of POD isozenzyme bands decreased in activity in the young leaves and flower buds as the ploidity decreased and this indicated that the gene dosages appeared to negatively correlate with the zymogram expressions of the POD isoenzymes.

关 键 词：同工酶 Cucumis属 种间杂交 异源四倍体 

分 类 号：Q789[生物学—分子生物学]