胸腺修饰诱导大鼠心脏移植耐受与白细胞介素-2,10的关系  

The relations between interleukin-2,10 and transplantation tolerance to rat cardiac allografts induced by intrathymic inoculation

作　　者：叶文学[1] 沈振亚[1] 于曙东[1] 余云生[1] 焦鹏[1] 

机构地区：[1]苏州大学第一附属医院心血管外科,215006

出　　处：《中华实验外科杂志》2006年第3期309-311,共3页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基因资助项目(39670719)

摘　　要：目的在手术当天进行胸腺修饰,诱导大鼠同种心脏移植免疫耐受,并对其可能机制作初步分析。方法通过胸腺注射和围手术期短程使用FK506来诱导心脏移植耐受,观察供心存活天数、混合淋巴细胞反应及受体鼠血清中白细胞介素(IL)-2、IL-10水平的变化。结果无处理组、对照组、经典诱导组和实验组供心存活时间分别为(6．8±1．9)、(17．4±5．1)、(73．8±8．6)、 (55．0±24．7)d,实验组与经典诱导组比较差异无统计学意义(P>0．05)。无处理组、经典诱导组和实验组的供受体脾细胞混合淋巴细胞培养刺激效应分别为198．72％、95．80％、67．94％,实验组和经典诱导组较无处理组增殖反应均明显降低(P<0．05),而两者间增殖反应差异无统计学意义 (P>0．05)。IL-10水平在无处理组移植心脏被排斥时为(48．10±5．14)ng／L较移植前(52．60± 10．14)ng／L差异无统计学意义(P>0．05);而在实验组早期呈低水平表达,为(36．10±2．30)ng／ L,术后中期(281．80±65．44)ng／L晚期(80．90±12．39)ng／L较移植前水平高得多,差异有统计学意义(P<0．05)。受体IL-2水平在无处理组发生排斥时为(159．80±59．19)ng／L较移植前 (54．80±8．42)ng／L明显升高,差异有统计学意义(P<0．05)。结论心脏移植手术当日胸腺内注射供体同种抗原,与术前21 d胸腺注射的经典诱导组同样能诱导宿主对移植物的低反应状态;IL-2 的水平与排斥反应的发生有关,而IL-10可能是免疫耐受的特异性指标,IL-10更可能与免疫耐受的维持有关。Objective To induce tolerance to cardiac aUograits by combining intrathymic inoculation with aUogeneic antigen at the day of transplantation. Methods Survival time of cardiac allografts（MST）, interlenkin （IL-2 and IL-10） in recipient＇s serum, the mixed lymphocyte reaction （MLR） between donors and recipients were investigated by inducing tolerance with short-course therapy of FKS06 and intrathymic inoculation. Results The MST of cardiac allografts in no treatment group, control group, classical induction group and experimental group was （6.8 ± 1.9 ）, （ 17.4 ± 5.1 ）, （73.8 ± 8.6） and （55.0 ± 24.7） days respectively. There was no significant difference between the classical induction group and experimental group （P 〉 0.05）. The proliferating response of MLR in no treatment group, classical induction group and experimental group was 198.72 %, 95.80 % and 67.94 % respectively. The proliferating responses in classical induction group and experimental group were lower than in no treatment group. The proliferating response had no significant difference between the classical induction group and experimental group （P〉0.05）. There was no significant difference in level of IL-10 before and after-operation （52.60 ± 10.14） ng/L vs （ 48.10 ± 5.14） ng/L in no treatment group （ P 〉 0.05 ）. In the experimental group, the expression of IL-10 was down-regulated on early stage （36.10± 2.30） ng/L and strongly up-regulated on medium （281.80± 65.44） ng/L and late stage （80.90± 12.39） ng/L after grafting. Before the grafts rejected in no treatment group, the level of IL-2 （159.80 ± 59.19） ng/L was higher than before heart transplantation [（54.80±8.42） ng/L, P〈 0. 05] . Conclusion Donor-specific tolerance was obtained by intrathymical injection of donor spleen cells on the day of transplantation as it was 21 days before transplantation. The level of IL-2 might be referable to occurrence of acute rejection and IL-10 might be relative to maintain immune

关 键 词：胸腺修饰 FK506 免疫耐受 心脏移植 

分 类 号：R654.2[医药卫生—外科学]