褐飞虱卵黄蛋白的分离及其生化特性  

Separation and characterization of vitellin of Nilaparvata lugens (Stl) (Homoptera: Delphacidae)

作　　者：戴华国[1] 衣维贤[1] 

机构地区：[1]南京农业大学植物保护学院,南京210095

出　　处：《昆虫学报》2006年第1期29-33,共5页Acta Entomologica Sinica

基　　金：国家自然科学基金项目(39970125)

摘　　要：电泳结合不同染色方法证实,褐飞虱Nilaparvatalugens(Stl)卵黄蛋白为一种糖脂结合蛋白,其分子量约为314kD,由148kD、124.5kD和39.6kD3个亚基组成。免疫反应证明,卵黄蛋白只存在于生育期的雌性褐飞虱成虫体内。褐飞虱卵黄蛋白具有种的特异性,其免疫血清与白背飞虱的卵黄蛋白无交叉反应。The vitellin of the brown planthopper （Nilaparvata lugens ） was assayed using polyacrylamide gel electrophoresis and different dyeing methods. The results indicated that the vitellin is a kind of protein containing sugar and fat. The molecular weight of the vitellin was 314 kD, consisting of three subunits with molecular weight of 148 kD, 124.5 kD and 39.6 kD respectively. Immunity experiment showed that the vitellin only existed in reproductive period of female adults of N. lugens. The vitellin of N. lugens was species-specific, and not reactive with the vitelhn of the white back planthopper （ Sogatella furcifera ）.

关 键 词：褐飞虱 卵黄蛋白 性质 免疫反应 

分 类 号：Q965[生物学—昆虫学]