p,p′-DDE对大鼠睾丸Sertoli细胞雄激素结合蛋白、转铁蛋白和抑制素B mRNA表达的影响  

Effects of p,p′-DDE on the Expression of Androgen-binding Protein,Transferrin and Inhibin B mRNA in Rat Sertoli Cells

作　　者：刘国红[1] 王翀[1] 刘宏凯[1] 杨克敌[1] 

机构地区：[1]华中科技大学同济医学院公共卫生学院,湖北武汉430030

出　　处：《中华男科学杂志》2006年第2期104-107,111,共5页National Journal of Andrology

基　　金：国家重点基础研究发展计划(国家973项目;2002CB512908)

摘　　要：目的:研究p,p′-DDE对大鼠睾丸Sertoli细胞雄激素结合蛋白(ABP)、转铁蛋白(Tf)和抑制素B(INH B)转录水平的影响。方法:建立Sertoli细胞体外培养模型,以不同浓度(10、30、50μmol/L)的p,p'-DDE染毒细胞,RT-PCR方法检测ABP、Tf和INH B mRNA的表达水平。结果:①随着p,p′-DDE染毒剂量的加大,引起ABP表达上调的同时Tf和INH B mRNA表达下调,且均呈剂量依赖性关系(P<0.05)。②ABP、Tf和INH B的相关性分析表明,ABP与Tf、INH B均呈负相关(r=-0.391 3,P=0.032 5;r=-0.235 2,P=0.015 8),而Tf和INH B呈正相关(r=0.451 6,P=0.004 7)。结论:p,p′-DDE对大鼠睾丸Sertoli细胞有生殖毒性作用,干扰ABP、Tf和INH B的转录水平,从而损伤支持细胞的功能,导致生殖障碍。Objective： To explore effects of p, p＇-DDE on the expression of androgen binding protein （ABP）, transferrin （Tf） and inhibin B （ INH B） mRNA in testis Sertoli cells of Sprague Dawley rats. Methods ： A method has been set up to obtain a large number of viable Sertoli cells from SD rats of 18-20 days of age. With a series of concentration p,p＇-DDE （ 10, 30, and 50μmol/L） co-incubating the Sertoli cells in vitro, the expression of ABP, Tf and INH B mRNA were determined by RT-PCR. Results： a） With increase of the incubated p, p＇-DDE, the expression of ABP mRNA in Sertoli cells went up while that of Tf and INH B dropped in a dose- dependent manner （P 〈 0.05 ）. b） The correlation analysis among ABP, Tf and INH B showed that negative relationships were found between ABP and Tf or INH B, respectively （r = -0. 391 3 ,P =0.032 5 ;r = -0. 235 2,P =0.015 8）, and that positive correlation was indicated between Tf and INH B （r =0.4516,P =0.0047）. Conclusion： p,p＇-DDE is a reproductive toxicant which disrupts the transcription of ABP, Tf and INH B in rat Sertoli cells so as to result in reproductive dysfunction.

关 键 词：2 2-双-(对氯苯基)-1 1-二氯乙烯 SERTOLI细胞 雄激素结合蛋白 转铁蛋白 抑制素B 

分 类 号：X503[环境科学与工程—环境工程]