银杏内酯B对血小板活化因子引起的巨噬细胞趋化及细胞骨架改变的影响(英文)  

Effect of ginkgolide B on the platelet-activating factor induced changes of chemotaxis and cytoskeleton of macrophages

作　　者：彭珊瑛[1] 张弗盈[1] 欧阳雪宇[1] 刘洋[1] 王文杰[1] 

机构地区：[1]中国医学科学院中国协和医科大学药物研究所,北京100050

出　　处：《药学学报》2006年第2期156-160,共5页Acta Pharmaceutica Sinica

基　　金：Hi-TechResearchandDevelopmentProgramofChina(2002AA233081)

摘　　要：目的考察银杏内酯B（ginkgolide B,BN52021）对血小板活化因子（PAF）引起的小鼠腹腔巨噬细胞趋化反应和丝状肌动蛋白（F-actin）聚合作用的影响.方法Boyden小室法检测化合物对巨噬细胞趋化的影响;流式细胞术检测特异性标记的巨噬细胞中F-actin的变化.结果PAF可显著刺激小鼠腹腔巨噬细胞产生趋化效应,PAF受体拮抗剂BN52021（0.01 nml·L^-1～0.1μmol·L^-1）可明显抑制该作用.此外,在含钙缓冲液中,BN52021可显著抑制PAF引起的丝状肌动蛋白聚合.结论BN52021可能通过抑制丝状肌动蛋白的聚合作用,从而抑制PAF引起的巨噬细胞趋化,并且这种作用是钙依赖性的.表明BN52021的抗炎作用途径之一是抑制PAF诱导的趋化作用.Aim To study the inhibitory effect of ginkgolide B （BN52021） on the PAF induced changes of chemotaxis of murine peritoneal macrophages and the related polymerization of F-actin. Methods Chemotaxis assays were performed using a modified 48-well Boyden chamber. Actin polymerization of murine peritoneal macrophages was analyzed by flow cytometry using a specific fluorescent stain. Results Peritoneal macrophages significantly migrated toward platelet-activating factor （PAF） through a micropore filter; however, in the presence of PAF receptor antagonist BN52021 （0. 01 nmol , L^-1 -0. 1 μmol · L^-1） , the migration was significantly inhibited. Moreover, BN52021 inhibited the actin polymerization of murine peritoneal macrophages induced by PAF in the presence of Ca^2＋ , but not in Ca^2＋ -free medium. Conclusion The results suggested that preventing polymerization of F-actin may be a pathway by BN52021 to inhibit the chemotaxis of macrophages, and this effect seems to be Ca^2＋ dependent. The data further indicated that inhibition of PAF induced macrophage chemotaxis is an imoortant mechanism underlying the anti-inflammatory action of BN52021.

关 键 词：趋化 丝状肌动蛋白 钙离子 腹腔巨噬细胞 银杏内酯B 

分 类 号：R282.71[医药卫生—中药学] R965[医药卫生—中医学]