藳本内酯和丁烯酉夫内酯对bFGF诱导的大鼠平滑肌细胞异常增殖的抑制作用(英文)  

Inhibitory effects of ligustilide and butylidenephthalide on bFGF-stimulated proliferation of rat smooth muscle cells

作　　者：梁明金[1] 贺浪冲[1] 

机构地区：[1]西安交通大学医学院药学系,陕西西安710061

出　　处：《药学学报》2006年第2期161-165,共5页Acta Pharmaceutica Sinica

基　　金：NationalScienceFoundationofChina(20075020,30371734)

摘　　要：目的研究藳本内酯和丁烯酉夫内酯与大鼠主动脉平滑肌细胞的亲合活性,及其对主动脉平滑肌细胞增殖的抑制作用。方法采用大鼠主动脉细胞膜色谱模型观察本内酯和丁烯酉夫内酯的保留特性;培养并分离纯化大鼠主动脉平滑肌细胞,采用bFGF诱导平滑肌细胞增殖,以MTT比色法检测本内酯和丁烯酉夫内酯对平滑肌细胞增殖的抑制作用。结果本内酯和丁烯酉夫内酯与大鼠主动脉平滑肌细胞膜有亲和性,其保留行为与钙离子受体拮抗剂维拉帕米相似;本内酯和丁烯酉夫内酯不会引起正常大鼠主动脉平滑肌细胞增殖,但能明显抑制bFGF诱导的大鼠主动脉平滑肌细胞的增殖。其有效浓度分别为5.5和11.1μmol.L-1(P<0.05)。结论本内酯和丁烯酉夫内酯与大鼠主动脉平滑肌细胞具有亲和力,能抑制血管平滑肌细胞的异常增殖。Aim To investigate the bio-affinities of ligustilide and butylidenephthalide to rat aortic smooth muscle cells and the inhibitory effects of them on bFGF-stimulated proliferation of rat vascular smooth muscle cell （VSMC）. Methods VSMCs were cultured from rat aorta pectoralis and identified by an immunohistochemical method. The bio-affinities between solute （ligustilide or butylidenephthalide） and cell membrane were measured by rat aortic cell membrane chromatography （CMC）. The inhibitory effects of ligustilide and butylidenephthalide on bFGF-stimulated VSMC proliferation were evaluated by MTT colorimetric method. Results Both ligustilide and butylidenephthalide had selective affinities to rat aortic smooth muscle cell as the same as verapamil, one of the calcium ion antagonists. They could potently separately （P ＜ 0.05 ）, but had no effects on the normal VSMC growth. Conclusion Both ligustilide and butylidenephthalide can inhibit the abnormal proliferation of VSMC induced by bFGF.

关 键 词：藁本内酯 丁烯酜内酯 细胞膜色谱法 细胞增值 平滑肌细胞 碱性成纤维细胞生长因子 

分 类 号：R965[医药卫生—药理学] R972[医药卫生—药学]