羟乙基葛根素对脑星形胶质细胞氧化性损伤的保护作用  

Protective effects of hydroxyethylpuerarin against brain astrocytes injury induced by hydrogen peroxide

作　　者：张斌[1] 魏欣冰[1] 刘慧青[1] 王立祥[1] 孙茹[1] 张岫美[1] 

机构地区：[1]山东大学医学院药理学研究所,山东济南250012

出　　处：《药学学报》2006年第2期171-174,共4页Acta Pharmaceutica Sinica

摘　　要：目的研究羟乙基葛根素对大鼠脑星形胶质细胞氧化性损伤的保护作用。方法取第4代培养的星形胶质细胞,以比色法测定细胞培养液中乳酸脱氢酶(LDH)活性,流式细胞术测定细胞凋亡率,[3H]-谷氨酸摄取法测定细胞摄取功能,比色法测定细胞内超氧化物歧化酶(SOD)活性及丙二醛(MDA)含量。结果羟乙基葛根素可明显降低过氧化氢(H2O2)损伤所致的星形胶质细胞LDH的释放、降低细胞凋亡率、增加谷氨酸摄取率、使细胞内MDA含量减少而SOD活性增加。结论羟乙基葛根素可改善星形胶质细胞的神经营养功能、抑制星形胶质细胞凋亡,其机制可能与其抗氧化作用有关。Aim To study the protective effects of hydroxyethylpuerarin against the injury of astrocytes induced by hydrogen peroxide（H2O2 ）. Methods Experiments were performed with cells from passage 4. Plasma membrane integrity was measured by lactate dehydrogenase （LDH） release. The occurrence of apoptosis was measured by flow cytometry. The glutamate uptake of astrocytes was studied with [ ^3 H ] -glutamate incorporation. Intracellular superoxide dismutase （SOD） activity and malondialdehyde （MDA） level were assessed by automatic biochemistry analyzer. Results Compared with H2O2 injured group, the occurrence of apoptosis, levels of LDH release and intracellular MDA of astrocytes reduced in hydroxyethylpuerarin pre-treated groups, but the glutamate uptake and intracellular SOD activity of astrocytes increased. Conclusion Hydroxyethylpuerarin could reduce the occurrence of apoptosis and improve neurotrophic function of astrocytes , which may be related with its antioxidant effects during oxidative stress.

关 键 词：星形胶质细胞 氧化应激 细胞凋亡 羟乙基葛根素 

分 类 号：R282.71[医药卫生—中药学] R963[医药卫生—中医学]