脐带血采集新方法的研究  

Study on the Collecting method of umbilical cord blood

作　　者：蔡文智[1] 于秀良[1] 陈翠华[1] 

机构地区：[1]第一军医大学南方医院

出　　处：《中华护理杂志》1996年第1期5-7,共3页Chinese Journal of Nursing

摘　　要：对脐带血采集新方法的研究旨在为研究脐带血造血细胞移植的可行性和为临床造血干细胞移植的来源服务。实验结果显示采用改良的封闭式采血法（优化法）收集脐带血。血量均可达１２０ｍｌ以上，平均１３２．２±１２．１３ｍｌ，最多一份达１５８ｍｌ。病原学检测结果均为阴性，无凝集现象发生。同时检测单份脐带血造血细胞数量为：有核细胞１８．９×１０±１７×１０，单个核细胞８．２×１０±０．８×１０。故采用优化法是一种可取的好方法，其脐带血采集量和单份脐带血造血细胞数量可达到移植所需的范围。而保证脐带血数量和质量的关键是及早断脐和及时穿刺脐带血管。The methods of cord blood collection was investigated in this study.The purpose of this study was to evaluate human umbilical cord blood as a alternative to bone marrow in the provision of transplantable progenitor cells for hematopoietic reconstitution.The results showed that using modified technique with a sterile and closed system,the volum of cord and placental blood would all exceed 120ml.The averge amount of blood collected wae 132.2±12.13ml and the highest was 158ml.Cord blood culture for bacteria and fungi were negative.Blood clots were not present.And in the exmination of each single cord blood,the contents of nucleated cells(NS) and mono-nucleated(MNS) were,respectively,18.9×10±1.7×10,8.2×10±0.8×10,The result suggested that the collection of placental.blood with the modified technique in a sterile and closed system is a simple,safe and efficient procedure.The average amount of blood collected and hematopoietic cells would satisfy the needs of cord blood trasplantation. Immediate cut of the umbilical cord followed by a prompt puncturing is critical for the volume and quality of the sample.

关 键 词：脐带血 造血细胞 采集方法 

分 类 号：R472.9[医药卫生—护理学]