神经干细胞体外增殖分化的钙成像研究  

CALCIUM IMAGING OF in-vitro PROLIFERATION AND DIFFERENTIATION OF NEURAL STEM CELLS

作　　者：刘一夫[1] 左皓[1] 周畅[1] 文梅梅[1] 谢佐平[1] 

机构地区：[1]清华大学生物科学与技术系,北京100084

出　　处：《生物物理学报》2006年第1期27-33,共7页Acta Biophysica Sinica

基　　金：国家自然科学基金项目(30370468)~~

摘　　要：神经干细胞具有广阔的应用前景,但对于其增殖和分化的内源机制、外部环境信号还并不十分了解。研究表明,钙信号很可能在其中起到了调控作用。利用钙离子成像技术,观察神经干细胞的单细胞体外增殖和分化过程,记录了在细胞分裂过程中钙信号变化的曲线。发现细胞增殖和分化过程中都会产生钙浓度的变化,但在细胞分裂后期两者钙信号的模式却存在差别。实验结果提示,胞内钙水平的波动只是细胞增殖的伴随产物,但却是细胞分化的必要条件。由此提出钙信号对神经干细胞分化调控机制的假设,并指出其对今后研究的意义。Neural stem cells（NSCs）, for their multipotency, have become a promising star in cell replacement therapy. However, their clinical use is restricted because of the poor knowledge of the intracellular and extracellular dues contributing to the proliferation and differentiation of NSCs. Recent studies showed that Ca^2＋ signals may regulate these processes. In this research, calcium imaging technique was used to observe the process that single NSC proliferated and/or differentiated in vitro. The results implied that the calcium signals in NSCs were appeared in both proliferation and differentiation process, and in proliferation, calcium transients are the accompanying signals reflecting cellular events, while in differentiation, the dependent signals associated with cell fate tightly. Eventually, it hypothesized a possible mechanism that how Ca^2＋ signals regulate the process of the differentiation of NSCs and pointed out the perspective of future research.

关 键 词：神经干细胞 钙信号 增殖和分化 钙离子成像 

分 类 号：Q421[生物学—神经生物学]