体外诱导人骨髓间充质干细胞定向血管内皮细胞分化  

In vitro Differentiation of Human Bone Marrow-derived Mesenchymal Stem Cells into Blood Vessel Endothelial Cells

作　　者：梁峰 王韫芳[2] 南雪[2] 岳慧敏[2] 徐迎新 施双双[2] 李荣 裴雪涛[2] 

机构地区：[1]Department of General Surgery,General Hospital of PLA [2]军事医学科学院野战输血研究所干细胞与再生医学研究室,北京100850

出　　处：《中国医学科学院学报》2005年第6期665-669,i0005,共6页Acta Academiae Medicinae Sinicae

基　　金：国家重点基础研究发展规划项目(973项目)(2001CB509906);国家高技术研究发展计划项目(863项目)(2002AA205051;2003AA205160)~~

摘　　要：目的研究人骨髓间充质干细胞(BDMSC)向血管内皮细胞的诱导分化,为复杂器官组织工程血管化及细胞移植修复损伤组织提供理想的细胞来源。方法以血管内皮细胞生长因子、碱性成纤维细胞生长因子对种植于纤维蛋白凝胶及基底膜基质中的BDMSC进行三维立体诱导培养,在培养后不同时间分别进行形态观察、组织切片和CD34、CD31、血管内皮生长因子受体-1(VEGFR-1,Flt-1)、VEGFR-2(Flk-1)、vWF的免疫组织化学荧光染色。结果诱导后的人BDMSC阳性表达血管内皮细胞特有表面标志CD34、CD31、Flt-1、Flk-1、vWF,并生成内皮样细胞,形成血管样结构。结论血管内皮细胞生长因子、碱性成纤维细胞生长因子及诱导后表达的Flt-1、Flk-1等表面分子可能在BDMSC向血管内皮细胞分化、并形成血管样结构的过程中提供适宜的微环境并起重要作用,这为间充质干细胞在组织工程血管化及细胞移植修复损伤中的应用提供了理论与技术上的支持。Objective To study differentiation of human bone marrow-derived mesenchymal stem cells （BDMSC） into blood vessel endothelial cells for ideal cell origin of complex organ tissue engineering vascularization and injured tissue repairing by cell transplantation. Method After different days of induction with vascular endothelial growth factor （VEGF） and basic fibroblast growth factor （bFGF） in 3D fibrin-gels and matrigel, BDMSC and angiogenesis were determined by the utilization of morphological observation, tissue section and CD34, CD31, vascular endothelial growth factor receptor-1 （VEGFR-1, Fit-1）, VEGFR-2 （Flk-1）, and vWF that were special for blood vessel endothelial cells. Result After 3D-cultured and induced with VEGF and bFGF in vitro in fibrin-gels and matrigel for 3-21 days, BDMSC expressed CD34, CD31, Fit-1, Flk-1, and vWF came into vessel-like configuration. Conclusion VEGF, bFGF as well as Flt-1 and Flk-1, expressed by BDMSC, may form a feasible microenviroment after induction and play an important role during processes of blood vessel endothelial cell differentiation and vessel-like configuration forming of BDMSC. Mesenchymal stem cells may be applied to tissue engineering vascularization and injured tissue repairing by cell transplantation.

关 键 词：骨髓间充质干细胞 血管内皮细胞 基底膜基质 纤维蛋白凝胶 血管内皮细胞生长因子 碱性成纤维细胞生长因子 

分 类 号：Q813[生物学—生物工程]