丙酸睾丸酮对不同功能运动神经元群降钙素基因相关肽配布式样的影响  

Effect of Testosterone Propionate on the Distribution Pattern of Calcitonin Gene-related Peptide in Different Motoneuron Pools

作　　者：石葛明[1] 李双成 张凯 邢凌霄 崔慧先 

机构地区：[1]河北医科大学基础医学院神经生物研究室,石家庄050017 [2]Department of Human Anatomy,Institute of Basic Medical Sciences,Hebei Medical University

出　　处：《中国医学科学院学报》2005年第6期739-742,i0014,共5页Acta Academiae Medicinae Sinicae

基　　金：河北省自然科学基金(397254)~~

摘　　要：目的探讨丙酸睾丸酮对大鼠两种不同功能运动神经元群降钙素基因相关肽(CGRP)配布式样的影响。方法应用霍乱毒素B亚单位结合胶体金(CB-Au)逆行标记神经元复合免疫细胞化学反应的非荧光双标记法,揭示CB-Au标记运动神经元群的CGRP免疫反应配布式样及其改变。结果与相应正常对照组和去势组相比,肌肉注射丙酸睾丸酮28d后,快肌-趾长伸肌运动神经元群调质CGRP的表达显著降低(P<0.001);对慢肌-比目鱼肌运动神经元群CGRP样免疫反应的配布式样影响较小(P>0.05)。结论快肌-趾长伸肌运动神经元群比慢肌-比目鱼肌运动神经元群在CGRP表达调节上更易受到雄激素的影响。Objective To study the effect of testosterone propionate calcitonin gene-related peptide （CGRP） in two types of motoneuron （Mn） labeling （TP） on pools in the distribution pattern of rats. Method The double of cholera toxin B subunit coupled with colloidal gold （CB-Au） retrograde identification combining with immunocytochemistry was mainly used to reveal the distribution pattern of CGRP-like immunoreactivity （CGRP-LI） and its changes in the motoneuron pools labeled by CB-Au. Result TP injected intramuscularly 28 days later significantly decreased CGRP expression in Mn pool innervating extensor digitorum longus （EDL, fast-twitch）, comparing with corresponding control and castration group respectively （P 〈 0.001）, while no significant effect on Mn pools innervating soleus （SOL, slow-twitch, P 〉 0.05） was observed. Conclusion EDL-Mn pool is more sensitive to testosterone propionate than SOL-Mn pool in regulating CGRP expression.

关 键 词：丙酸睾丸酮 降钙素基因相关肽 运动神经元 

分 类 号：R322.81[医药卫生—人体解剖和组织胚胎学]