人破伤风类毒素免疫血浆筛选试验中两种抗体检测方法的比较  

Comparasion of two antibody detection methods in screening human antiserum for tetanus toxoid

作　　者：朱华松[1] 周志军[1] 尹斌[1] 李健[1] 

机构地区：[1]武汉生物制品研究所,武汉430060

出　　处：《微生物学免疫学进展》2006年第1期39-42,共4页Progress In Microbiology and Immunology

摘　　要：应用酶联免疫法(ELISA)和间接血凝法(IHA)对1014份破伤风类毒素全程免疫后人血浆进行抗体水平检测,比较两者的收浆率、收浆符合率以及与动物实验的相关性。结果表明两者收浆率均达80%。ELISA法与IHA法的合格浆符合率达92%,与动物实验的相关性更好。ELISA法操作简便快速,结果判读客观明确,优于传统的IHA法,可作为人破伤风类毒素免疫血浆筛选的常规方法。Detect antibody level by ELISA and IHA from 1014 antiserum samples of persons by complete tetanus toxoid vaccination, compare serum collecting rates and collecting rates in accordance with the standard and the correlation in between the two methods and animal test. The collecting rates for both methods are 80%. Conformation rate of ELISA to IHA is 92% and the correlation in between ELISA and animal test are better. ELISA can be rapidly and simply operated and the result can be directly and clearly determined. It is superior to IHA that ELISA could be used as a routine method for screening human antiserum for tetanus toxoid.

关 键 词：破伤风类毒素免疫血浆 ELISA IHA 收浆率 符合率 相关性 

分 类 号：R446.6[医药卫生—诊断学]