蝉拟青霉菌株间营养亲和性的初步研究  

Study on vegetative compatibility among isolates of Paecilomyces cicadae(Miquel)Samson

作　　者：赵杰宏[1] 刘爱英[1] 梁宗琦[1] 

机构地区：[1]贵州大学真菌资源研究所,贵州贵阳550025

出　　处：《山地农业生物学报》2006年第1期39-43,共5页Journal of Mountain Agriculture and Biology

基　　金：国家自然科学基金资助项目(30360064)

摘　　要：从云南、贵州两地采集的7个蝉拟青霉Paecilomyces cicadae(M iquel)Sam son菌株上分离出32个单孢子菌株,在含质量浓度20g.L-1氯酸盐的培养基上25℃左右培养5d,结果表明,菌落的多个角度出现不同形态特征的角变,可以分成3种类型。从这3种类型中选取了110个角变转接到MM培养基上观察其营养利用情况,最后共获得了抗氯酸盐的不利用硝酸盐突变菌株(n it突变体)60株,没有角变类型的专一对应关系。然后把这60株n it突变体在MM培养基上,25℃左右经配对组合培养21d进行营养亲和分析,结果显示,只有来自北回归线站云南墨江哈尼族自治县的7个n it菌株有营养亲和现象,其他菌株内或菌株间皆不亲和。菌落融合区菌丝经荧光染色观察,大量存在双核和多核细胞菌丝以及“H”型菌丝接合现象,未发现存在双核或多核分生孢子。Thirty-two single spore isolates were obtained from seven strains of Paecilomyces cicadae（Miquel） Samson that were collected from Yunnan and Guizhou provinces. On KPS containing 20g·L^-1 KClO3, several sectors appeared from different directions of one clone after incubating at 25℃ for five days. These sectors could be divided into three types. When 110 sectors were selected and inoculated on minimal medium, we obtained sixty nitrate nonutilizing（nit） mutants from them as compared with their morphology of clones. It showed that there was no special corresponding relation between nit mutants ann sector types. When Vegetative compatibility tests were carried out by pairing the sixty nit mutants on minimal medium at 25℃` for twenty-one days, only seven nit mutants collected from Mojiang of Yunnan province were vegetatively compatible while others showed negative reaction. The hypha in mating line stained by Hoechest 33258 showed that hypha with two or more nucleus and phenomenon of ＂ H＂ anastomosis were observed. But we didn＇ t find any conidia with two or more nuclei.

关 键 词：蝉拟青霉 nit突变株 营养亲和性 异核体 

分 类 号：S476.12[农业科学—农业昆虫与害虫防治]