葡萄糖与甲胺还原胺化产物的高效液相色谱分析  

The Analysis of Hydrogenation Products of Glucose and Methyl amine by High Performance Liquid Chromatographic

作　　者：刘娜[1] 张津枫[2] 高瑞昶[1] 张广明[2] 

机构地区：[1]天津大学化工学院,天津市300072 [2]天津天士力集团有限公司化学制药研究所

出　　处：《光谱实验室》2006年第1期35-38,共4页Chinese Journal of Spectroscopy Laboratory

摘　　要：建立了以C18柱、RI检测器,甲醇-水-三氟乙酸为流动相,葡丙胺作内标物,分析葡萄糖与甲胺还原胺化产物1-脱氧-1-(甲氨基)-D-山梨醇纯度的HPLC法。方法具有快速、准确、简便等特点,N-甲基葡糖胺、N-甲基葡糖苷、山梨醇的相对标准偏差分别为1．42％、3．24％、2．45％,回收率分别为95．2％- 100．9％、96．7％-123．0％、98．3％-117．0％,检出限分别为33ng、38ng、18ng。N-methyl glucamine and main impurities of N-methyl glucoseimine and sorbitol in hydrogenation of glucose and methylamine were well separated and determined by HPLC with a C18 column and internal standard method. Good liner relationships for N-methyl glucamine, N-methyl glucoseimine and sorbitol were observed with the correlation coefficients of 0. 99998, 0. 9999 and 0. 99989,RSD of 1.42%,3. 24% and 2. 45%,recovery of 95.2--100. 9%, 96. 7--123. 0%,and 98.3--117.0% and detection limit of 33,38, and 18ng, respectively.

关 键 词：1-脱氧-1-(甲氨基)-D-山梨醇 高效液相色谱 内标法 

分 类 号：O657.72[理学—分析化学]