胰岛素样生长因子－I　mRNA在前列腺组织中的表达  

The expression of insulin-like growth factor in prostatic tissues

作　　者：邓方明[1] 顾方六[1] 张丽英 赵小东 李伟雄 夏同礼[1] 孔祥田[1] 姜辉[1] 

机构地区：[1]北京医科大学第一医院,北京医科大学泌尿外科研究所,国家计划生育研究所,北京医科大学人民医院

出　　处：《中华泌尿外科杂志》1996年第4期235-237,共3页Chinese Journal of Urology

摘　　要：对１５例良性前列腺增生（ＢＰＨ）和１２例正常前列腺组织标本中胰岛素样生长因子－Ｉ－（ＩＧＦ－Ｉ）ｍＲＮＡ的表达情况进行了研究。ＲＴ－ＰＣＲ的方法可自所有标本中检出ＩＧＦ－ＩｍＲＮＡ的表达，并用电泳和Ｓｏｕｔｈｅｒｎ杂交证实了结果的可靠性。原位杂交表明１３／１５的ＢＰＨ和９／１２的正常前列腺标本中有ＩＧＦ－ＩｍＲＮＡ表达，ＩＧＦ－Ｉ在上皮的表达强于间质，尤以基底细胞表达最明显。ＩＧＦ－ＩｍＲＮＡ在ＢＰＨ组织中的量多于正常前列腺。In situ hybridization and reverse transcription PCR were adopted to map the insulin-like growth facto-I(IGF-I)gene expression in 15 benign hyperplastic and 12 normal prostatic tissues. IGF-I mRNA could be detected by RT-PCR in all the tissue samples,With in situ hybridization,13 of the 15 benign hyperplastic prostatic tissues and 9 of 12 normal prostatic tissue expressed the IGF-I mRNA. The latter was mainly located in the epithelial cells especialy in the basal cells and was expressed at low levels in the stromal cells,The level of expression in BPH was much higher than that in normal prostate suggesting that IGF-I might play an important role in the growth regulation of prostate and the pathogenesis of BPH.

关 键 词：前列腺增生 胰岛素样 生长因子 组织化学 

分 类 号：R697.32[医药卫生—泌尿科学]