一个新的人类乙酰转移酶基因hNATL的克隆与表达谱研究  

CLONING AND EXPRESSION ANALYSIS OF HUMAN N-ACETYLTRANSFERASE DOMAN CONTAINING GENE hNATL

作　　者：邹星[1] 慈宏亮[1,2] 陈微[3] 李亦平[1,2,3] 

机构地区：[1]北京师范大学生命科学学院生物医学研究所 [2]浙江赛尔生物医学研究院,杭州310006 [3]美国哈佛大学牙医学院Forsyth研究所

出　　处：《分子细胞生物学报》2006年第1期22-28,共7页Journal of Molecular Cell Biology

基　　金：浙江赛尔生物医学研究院资助项目。~~

摘　　要：从小鼠cDNA序列入手,依据同源分析的电子克隆方法得到一个包含N末端乙酰转移酶结构域的人类新基因hNATL(Human NAT Like)。hNATL的cDNA长1803bp,编码区621bp,Genbank 登陆号AY632082。hNATL编码的蛋白长206个氨基酸残基,包含有N乙酰转移酶结构域。hNATL 基因定位于人染色体17q25．2区,包括6个外显子和5个内含子。基因表达汇编分析结果显示, hNATL在人脑和生殖腺中高表达。Northern杂交显示,在成年小鼠中hNATL在心脏,脾脏和卵巢中出现表达。整体原位杂交的结果显示,hNATL特异表达于E7．5和E8．5的小鼠胚胎脑部和HH10期鸡胚胎的头部。上述结果提示,hNATL对胚胎期脑的发育和成体中重要器官行使正常功能发挥十分重要的作用。A novel human gene hNATL （Human NAT Like） was cloned by in silico cloning and RT-PCR. hNATL cDNA is 1803bp in length with a 621bp coding region, and the Genbank accession No. is AY632082. hNA TL encodes a protein of 206 amino acid residues which containing a N-acetyhransferase domain, hNATL is located on human chromosome 17q25.2 with 6 exons. Serial analysis of gene expression revealed that hNA TL was highly expressed in human brain and gonad, while hNA TL was expressed in heart, spleen and gonad of adult mouse. Whole-mount in situ hybridization showed that hNATL specifically expresses in E7.5 and E8.5 mouse embryo brains and in HH10 stage chicken embryo brain. These results suggest that hNATL may play an important role in the development of embryo brain and may also be important for function of adult brain and gonad.

关 键 词：N末端乙酰转移酶结构域 胚胎发育 整体原位杂交 

分 类 号：Q78[生物学—分子生物学]