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作 者:徐培洲[1] 李云[1] 张红宇[1] 韩磊[1] 汪旭东[1] 吴先军[1]
机构地区:[1]四川农业大学水稻研究所教育部西南作物基因资源与遗传改良重点实验室,成都611130
出 处:《分子细胞生物学报》2006年第1期66-70,共5页Journal of Molecular Cell Biology
基 金:教育部长江学者和创新团队发展计划(IRTO453)四川省青年基金四川省教育厅重点项目资助。
摘 要:水稻同源三倍体与二倍体之间的杂交曾有过报道,渡边等发现三倍体自交表现为完全不结实,若授以正常的二倍体花粉,结实率为13.5%。Sen在籼型品种T1242自然产生的三倍体与二倍体的杂交后代中,得到了三体、双三体和四体等。另外,Watanabe、Hu、Ramanujam、胡兆华和Qu等也进行过研究。但都是对其中出现的非整倍体进行研究,对于出现的二倍体.未有详细的报道。In this report,the process of hybridization between different ploidy levels was observed in rice. Autotriploid (2n=36) 149-B,generated naturally from the twin-seedling rice population SAR-2,was used as the female parent crossing with a normal diploid variety Shuhui 363. The processes of fertilization and embryological development were investigated. The pollen could germinate in triploid tissue of pistil normally and arrive at micropyle. But the percentage of double fertilization was only 8.89% ,and the percentage of seed setting was 0.566%. The hybridization obstacles mainly occurred in the process of fertilization. Barriers of double fertilization, prolongation and abnormal development of proembryo, and even development stopping or differentiation failure of embryo and/or endosperm could result in abnormal development and abortion of zygote.
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