分子佐剂C3d上调Raji细胞协同刺激分子B7-1和B7-2的表达(简报)  

MOLECULAR ADJUVANT C3d UP-REGULATES BOTH B7-1 AND B7-2 EXPRESSION ON Raji CELLS

作　　者：余敏[1] 李大金[1] 王秀丽[1] 袁敏敏[1] 朱影[1] 姚晓英[1] 李华萍[1] 

机构地区：[1]复旦大学附属妇产科研究所生殖免疫室,上海200011

出　　处：《分子细胞生物学报》2006年第1期77-82,共6页Journal of Molecular Cell Biology

基　　金：国家自然科学基金(30271235)上海市自然科学基金(00ZB14058)教育部博士学科点专项科研基金(9941)卫生部优秀青年人才专项科研基金(97031)资助。

摘　　要：我们在以往研究中，引入选择性增强体液免疫效应的新型分子佐剂C3d，成功构建了重组避孕疫苗hCGβ-C3d3，通过免疫Th2型优势的BALB／c小鼠和Th1型优势的C57BL／6小鼠．显示分子佐剂C3d在不同品系小鼠均使免疫效应从Th1型细胞免疫向Th2型体液免疫偏倚。To explore modulation of the molecular adjuvant C3d in the hCGβ-C3d3 fusion protein in costimulatory molecule expression on Raji cells by linking to the surface molecule CD21. Raji cells, B cell line, were incubated with the purified hCGβ-C3d3, hCGβ or C3d3 protein for 15 hours respectively in the interference of anti-CD21 monoclonal antibody or not. The culture concentration of each group was 10, 30, or 90μg/ml respectively. The expression of both B7-1 and B7-2 on Raji cells were analyzed by flow cytometric assay. It was observed that compared to hCGβ or C3d3 protein, hCGβ-C3d3 up-regulated both B7-1 and B7-2 expression on the Raji cells, and the effect was in a dose-dependent manner. The up-regulation effect was blocked efficiently by anti-CD21 mAb. The results showed that molecular adjuvant C3d may up- regulate both B7-1 and B7-2 expression on Raji cell via C3d/CD21/CD19 complex, thus enhance the antigen presentation of B cell, and the interaction between B cell and T cell.

关 键 词：分子佐剂C3d HCGΒ B7—1 B7-2 

分 类 号：R392.1[医药卫生—免疫学]