影响农杆菌介导的麻疯树基因转化因素的研究(简报)  

STUDY ON FACTORS INFLUENCING AGROBACTERIUM-MEDIATED TRANSFORMATION OF JATROPHA CURCAS

作　　者：李美茹[1] 李洪清[2] 吴国江[1] 

机构地区：[1]中国科学院华南植物研究所,广州510650 [2]华南师范大学生命科学学院,广州510631

出　　处：《分子细胞生物学报》2006年第1期83-89,共7页Journal of Molecular Cell Biology

基　　金：中国科学院知识创新工程重要方向项目(No:KSCX2-SW-130)中科院百人计划资助。

摘　　要：麻疯树（Jatropha curcas）为大戟科麻疯树属植物，是一种多年生木本油料植物。原产美洲，广泛分布于热带亚热带地区。因其种子含油量高达60％．可作生物燃料之用，麻疯树是目前正在被开发利用的重要能源植物之一。除此之外，麻疯树的种子含有多种活性成分，有着重要的农药和医药价值；其生长能耐干旱贫瘠，可用于荒山造林。因此，麻疯树是一种具有多种用途、重要经济价值和学术研究价值的植物。目前对其研究多停留在植物组织培养、植物化学、毒理学、种植业方面等。Susceptibility of explants to antibiotics and the transient expression of β- glucuronidase （GUS） gene were studied to determine the optimal conditions for gene transformation of Jatropha curcas mediated by Agrobacterium Tumefaciens. The results showed that Jatropha curcas was insensitive to the antibiotic cefotaxime, which is used for suppressing the growth of Agrobacterium after cocuhivation, while the plant was sensitive to kanamycin, hygromycin, phoshpinothrincin. All the three selective reagents synchronously suppressed the induction and the growth of callus, shoot organogenesis in a dose-dependent manner. The types of explants and the physiological state of the explants had strong influence on the transient expression efficiency of GUS. Hypocotyl, cotyledon petiole and cotyledon from 14-day-old seedling were more susceptible to Agrobacterium infection. Hygromycin-resistance calli from cotyledon explants were obtained and showed GUS staining.

关 键 词：麻疯树 农杆菌 基因转化 β-葡糖醛酸酶(GUS)基因 

分 类 号：Q949.9[生物学—植物学] Q943.2