中国女性血细胞X染色体失活偏移与年龄相关  

X-chromosomal inactivation skewing in blood cells in Chinese healthy females screened for the females predisposed to cancer.

作　　者：李刚[1] 苏勤[1] 张伟[1] 刘贵秋[1] 巩丽[1] 朱少君[1] 张贺龙[2] 冯英明[2] 

机构地区：[1]第四军医大学唐都医院病理科,陕西西安710038 [2]第四军医大学唐都医院肿瘤科,陕西西安710038

出　　处：《现代肿瘤医学》2006年第3期266-269,共4页Journal of Modern Oncology

基　　金：国家自然科学基金资助项目(No.30572125和No.30171052);陕西省卫生厅科研基金项目(02D02)

摘　　要：目的 了解健康中国女性个体X染色体失活偏移（SXCI）的发生状况，明确年龄因素是否与之关联。方法 采集401例健康中国女性的外周血标本，提取基因组DNA，经HpaⅡ消化后，PCR扩增雄激素受体（AR）基因，产物经变性聚丙烯酰胺凝胶电泳，银染显示结果，比较HpaⅡ消化前后等位基因扩增产物的强度差异，计算其校正比值（CR）。结果 无论采用CR≥3抑或≥10作为标准，老年组（50岁～96岁）SXCI发生率均显著高于新生儿组（出生后4周以内）或青年组（16岁～50岁），而新生儿组与青年组之间差异不显著。采用标准CR≥3时，新生儿组为4．3％（P=0．532vs青年组），青年组为7．8％（P=0vs老年组），老年组为25．7％（P=0．001vs新生儿组）。采用CR≥10标准时，新生儿组为2．2％（P=1vs青年组），青年组为1．2％（P=0vs老年组），老年组为11．1％（P=0vs新生儿组）。结论 健康中国女性SXCI发生率是随年龄的增长而变动的，年龄与女性个体SXCI的发生相关。在筛选女性肿瘤易感个体时，应注意用作对照的健康女性的SXCI的发生状况。Objective To investigate whether age is associated with X- chromosomal inactivation skewing in blood cells in Chinese healthy females . Methods DNA was extracted from mononuclear blood cells from 401 Chinese healthy females. Exon 1 of androgen receptor （AR） gene was amplified, with its products from different alleles resolved on denaturing polyacrylamide gels and visualized by silver staining. Corrected ratio （ CR ） of the AR allele for each HpaⅡ digested and auto - control sample pair was calculated. All statistical tests were two - sided. Results Comparisons were made among three age groups： ① neonates, ②young women group of females 16 to 50 years old and, ③old women group of females 50 to 96 years old. With CR≥3 adopted as the criterion, the incidence of skewing was 4.3% （ 2 of 46） in neonates（P =0. 532 vs young women group） ,7.8% （ 13 of 166） in young women group（P = 0vs old women group）, and 25.7% （ 37 of 144） in old women group （P = 0. 001 vs neonates）. When a more stringent criterion of CR≥ 10 for skewing was used, the incidence of skewing was 2.2% （ 1 of 46） in neonates （P=1vs young women group） ,1.2% （ 2 of 166） in young women group（P =0vs old women group） ,and 11.1% （ 16 of 144） in old women group（P=0vs neonates）. Conclusion Age is associated with X -chromosomal inactivation skewing in blood cells in Chinese healthy females. More attention should be paid to X - chromosomal inactivation skewing in blood cells in Chinese healthy females screened for the ones predisposed to cancer.

关 键 词：X染色体失活 偏移 雄激素受体基因 女性 

分 类 号：R730.3[医药卫生—肿瘤]