核结合因子促进骨髓间质细胞MBA-1凋亡  

Apoptosis of mesenchymal cell line MBA-1 induced by core binding factor alpha 1

作　　者：刘敏[1] 周后德[1] 何玉玲[1] 谢辉[1] 廖二元[1] 

机构地区：[1]中南大学湘雅二医院代谢内分泌研究所,长沙410011

出　　处：《中南大学学报（医学版）》2006年第1期14-18,共5页Journal of Central South University ：Medical Science

基　　金：国家自然科学基金(30400218)

摘　　要：目的:研究核结合因子(core b ind ing factor alpha 1,Cbfa1)的2种亚型Cbfa1/P56和Cbfa1/P57对骨髓间质细胞MBA-1凋亡的影响,并探讨其促进MBA-1凋亡的作用机制。方法:用带有Cbfa1/P56或Cbfa1/P57全长cDNA的质粒瞬时转染到骨髓间质细胞MBA-1,72 h后用流式细胞仪观察细胞凋亡率的变化,并抽提蛋白进行Cbfa1及相关凋亡蛋白Bax,Bc l-2,cytochrom e-C,caspase-3,caspase-9和TNF-α的免疫印迹检测。结果:流式细胞仪检查发现,转染Cbfa1/P56或Cbfa1/P57质粒后,MBA-1细胞的凋亡率明显增加。W estern b lot显示,Cbfa1/P56或Cbfa1/P57能增加Bax蛋白表达和Bax/Bc l-2比值,并增加cytochrom e-C,caspase-3,caspase-9,TNF-α蛋白的表达。结论:Cbfa1/P56和Cbfa1/P57能诱导骨髓间质细胞MBA-1凋亡。Cbfa1促进MBA-1凋亡的机制包括线粒体途径和膜受体途径2种,凋亡相关蛋白Bax,Bc l-2,cytochrom e-C,caspase-9和caspase-3组成的信号级联反应构成了Cbfa1促进MBA-1凋亡的信号转导通路,TNF-α也参加了该凋亡的信号转导。Objective To investigate the effect of two core binding factors alpha 1 （ Cbfa1 ） isfroms （Cbfa1/P56 and Cbfa1/P57） on the apoptosis of mesenchymal cell line MBA-1. Methods The two Cb-fa1 isfroms were transiently transfected into MBA-1 cells, then the changes of apoptosis rate were observed by flow cytometer. The protein expressions of Cbfa1, Bax, Bcl-2, caspase-3, and caspase-9, cytochrome-C and TNF-α were determined by Western immunoblot. Results After the transient transfection with the two isforms of Cbfa1, MBA-1, the cells apoptotic rates increased, and the ratio of Bax/Bcl-2, the expressions of cytochrome-C, caspase-3, caspase-9 , and TNF-α were significantly increased. Conclusion Cbfa1 can promote the apoptosis in mesenchymal cell line MBA-1. Bax/Bcl-2, cytochrome-C, caspase-9 , caspase-3, and TNF-α are also involved in the apoptosis pathway.

关 键 词：核结合因子 细胞凋亡 信号传导通路 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]