肠球菌表面蛋白基因与生物膜形成关系的探讨  

Relationship of esp and biofilm formation in enterococci

作　　者：马立艳[1] 许淑珍[1] 

机构地区：[1]首都医科大学附属北京友谊医院,北京100050

出　　处：《山东医药》2006年第5期11-12,共2页Shandong Medical Journal

基　　金：北京市自然科学基金资助项目(7012013)

摘　　要：目的探讨肠球菌表面蛋白(esp)基因与生物膜形成之间的关系,以利临床采取预防措施和选药治疗。方法应用PCR方法检测145株临床分离肠球菌中esp的携带率,并用斑点杂交确证。用96孔聚苯乙烯板进行生物膜形成试验。结果96株粪肠球菌和49株屎肠球菌中esp的检出率分别为34.4%和36.7%;生物膜形成率分别为93.8%和91.8%;esp阳性的肠球菌生物膜形成率为100%。结论肠球菌普遍具有形成生物膜的能力,但生物膜的形成并不仅限于携带esp的肠球菌。Objective： To investigate the relationship of esp and biofilm formation in enterococci. Methods： PCR and dot blot were used to detect the incidence of esp in 145 clinical isolates. And the biofilm formation was performed in 96 well polystyrene plate. Results： In 96 E. faecalis and 49 E. faecium, the detection rate of esp was 34.4% and 36.7% respectively, hiofilm formation was 93.8% and 91.8% respectively; all the esp positive strains can form biofilm. Conclusions： Biofilm is much common among enterocoeci, hut the hiofilm formation exists not only in esp postive enterococci.

关 键 词：肠球菌 肠球菌表面蛋白 生物膜 

分 类 号：R318.02[医药卫生—生物医学工程]