偏最小二乘同步荧光光谱法同时测定鳗鱼组织中三种喹诺酮药物残留量  

Synchronous Fluorimetric Determination of the Residues of Three Quinolones in Eel by Partial Least Squares

作　　者：张国文[1] 倪永年[2] 

机构地区：[1]南昌大学食品科学教育部重点实验室,江西南昌330047 [2]南昌大学化学系,江西南昌330047

出　　处：《光谱学与光谱分析》2006年第1期113-116,共4页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金(20365002;20562009);江西省自然科学基金(0320014);江西省教育厅(赣教技字(2005)号)资助项目

摘　　要：应用偏最小二乘(PLS)算法对同步荧光光谱严重重叠的诺氟沙星、盐酸洛美沙星和乳酸左氧氟沙星药物三组分混合体系进行波谱解析,建立了该混合体系含量同时测定的新方法。在pH2.87BR缓冲溶液中,波长差Δλ=190nm时,诺氟沙星、盐酸洛美沙星和乳酸左氧氟沙星的测量线性范围分别为0.016～0.40μg·mL-1,0.01～0.336μg·mL-1和0.01～0.336μg·mL-1;检出限分别为0.0126,0.006和0.0072μg·mL-1。采用PLS法结合同步荧光法对鳗鱼样品进行测定,结果令人满意。In the present paper, the synchronous fluorimetric spectra of norfloxacin, lomefloxacin chlorhydric acid, and levofloxacin lactate with Δλ= 190 nm in a B-R buffer medium of pH 2.87 were measured, and the partial least squares （PLS） method was applied to the quantitative resolution of the seriously overlapped fluorimetric spectra of these compounds. The linear ranges for norfloxacin, lomefloxacin chlorhydric acid, and levofloxacin lactate are 0.016-0.40 μg·mL^-1, 0.01-0.336μg·mL^-1 and 0.01-0.336 μg·mL^-1 , respectively. The limits of detection are 0.012 6 μg·mL^-1 for norfloxacin, 0.006 μg· mL^-1 for lomefloxacin chlorhydric acid, and 0.007 2 μg·mL^-1 for levofloxacin lactate. The analytical results by PLS were compared with principal components regression （PCR） and classical least squares （CLS）, and this method was applied to the determination of these three compounds in eel samples with satisfactory results.

关 键 词：偏最小二乘 同步荧光光谱法 喹诺酮 

分 类 号：O657.3[理学—分析化学]