疣荔枝螺微粒体细胞色素P450酶系的光谱分析  

Spectral Analysis and Study of the Microsome Cytochrome P450 Aromatase of Thais clavigera

作　　者：韩雅莉[1] 邓瑞鹏[2] 周小朋[2] 

机构地区：[1]广东工业大学轻工化工学院,广东广州510090 [2]汕头大学生物系,广东汕头515063

出　　处：《光谱学与光谱分析》2006年第1期130-133,共4页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金(30271033)资助项目

摘　　要：从疣荔枝螺内脏团制备得到微粒体,用UVmini1240分光光度计对细胞色素P450酶系的三种主要成分:细胞色素P450、细胞色素b5,NADPH细胞色素P450还原酶进行了测定,并对微粒体酶液进行SDS-PAGE电泳测试,证明了疣荔枝螺体内含有细胞色素P450酶系的三种酶。The microsomes were prepared from the viscera masses of Thais clavigeras. It is well known that there are mine main components in microsome cytochrome P450 aromatase, including cytochrome P450, cytochrome b5 and NADPH-cytochrome P450 reductase. So the contents of cytochrome P450 and cytochrome b5, and the activity of NADPH-cytochrome P450 reductase were determined by Shimadzu UV Visible spectrophotometer. And the mierasomal proteins were analysed by SDS-PAGE.All the results indicated that there exists P450 arom in the T. clavigeras.

关 键 词：疣荔枝螺 微粒体 细胞色素P450酶系 

分 类 号：Q559.9[生物学—生物化学]