人芽囊原虫在RPMI1640培养基中培养条件的优化  

The Optimization of in Vitro Culture Conditions of Blastocystis hominis in RPMI 1640 Mediums

作　　者：答嵘[1] 乔继英[1] 王伟[2] 成少利[3] 卢朝辉[1] 张旭[1] 

机构地区：[1]西安交通大学医学院免疫与病原生物学系,西安710061 [2]西安交通大学第一医院神经外科,西安710061 [3]西安交通大学医学院形态实验中心,西安710061

出　　处：《热带医学杂志》2006年第2期141-144,164,共5页Journal of Tropical Medicine

基　　金：卫生部科学研究基金(No.98-1-231)

摘　　要：目的优化人芽囊原虫(Blastocystishominis)在RPMI1640培养基中的培养条件。方法将B.hominis接种至RPMI1640培养基中,观察酸碱度、接种量、血清种类、浓度、温度、空气中氧与粪便标本的选择对B.hominis生长的影响。结果在RPMI1640培养基中,pH7.5、接种量200000细胞/管、20%小牛血清,37℃,加入青霉素、链霉素为最佳培养条件。结论在RPMI1640中,接种以B.hominis阳性(颗粒型为主)的血粘液便,加入20%小牛血清,青霉素、链霉素,在pH7.5于37℃条件下厌氧培养,每6d转种一次,可达到长期培养B.hominis的目的。Objective To optimize in vitro culture conditions of Blastocystis hominis in RPMI1640 medium. Method The acidity, inoculation-dose, sorts and concentration of serum, temperature, oxygen, and selection of stool specimens were studied. Result pH 7.5,2×10^5 ceU/tube,20% bovine serum, 1×10^5 IU penicillin and 1.6×10^5 IU streptomycin,37℃ are the heat in vitro culture conditions for Blastocystis hominis. Conclusion Mucous and bloody stool specimens that contained mainly granular forms is a better source of Blastocystiis hominis for the culture. The optimal condition for culturing Blastocystiis hominis is in RPMI1640 at pHT.5 with 20% calf serum, streptomycin, and penicillin, under anaerobic condition at 37℃. Blastocystis hominis can culture for long time with subculture at every 6 days.

关 键 词：人芽囊原虫 RPMI1640培养基 培养条件 优化 

分 类 号：R382.9[医药卫生—医学寄生虫学]