环胞霉素A对一氧化氮生成作用的影响  

The Effects of Cyclosporin A on Nitric Oxide Synthesis in Response to Tumor Necrosis Factor α

作　　者：李声宏[1] 程绪杰[1] 李红霞[1] 

机构地区：[1]苏州大学附属第一医院血液净化中心,江苏苏州215006

出　　处：《中国血液流变学杂志》2005年第4期529-531,共3页Chinese Journal of Hemorheology

摘　　要：目的研究环胞霉素A对一氧化氮生成作用的影响。方法培养血管内皮细胞ECV304,分为TNFα组和TNFα+CsA组,TNFα刺激浓度为50ng/mL,CsA浓度为0.1、1、10μmol/L,培养24h后测定上清中的NO浓度;临床研究中的20例膜性肾病患者分为治疗组14例和对照组6例,治疗组服用CsA5mg/kg/d和强的松1mg/kg/d,对照组强的松1mg/kg/d,治疗4周,分别测定治疗前后外周血中的NO含量和24小时尿蛋白量。结果培养细胞研究发现TNFα组NO为74.63±5.83μmol/L,TNFα+CsA(0.11、、10μmol/L)组依次为11.49±1.72μmol/L9、.78±1.05μmol/L9、.54±0.83μmol/L,二者之间存在明显差异,P<0.01;14例膜性肾病患者治疗前后血NO浓度分别为197.58±21.64μmol/L、125.39±16.46μmol/L,二者之间存在明显差异,P<0.01,而对照组治疗前后为186.72±15.43μmol/L和179.45±18.32μmol/L,没有明显差别,治疗组4周前后的24小时尿蛋白量分别为4.53±0.92g和3.81±1.26g,P<0.01,而对照组前后变化无统计学意义。结论环胞霉素A可以抑制TNFα诱导的NO产生。Objective To investigate the effects of cyclosporin A on nitric oxide synthesis induced by TNF1^1A. Method Cultivated ECV304 cells were stimulated by TNF α （50ng/mL） or TNF α （50ng/mL） plus CsA（0.1,1,10μ mol/L）.NO metabolites of supernatant was detected.ln clinical study fourteen patients with membranous nephropathy accepted four weeks treatment of cyclosporin A（5mg/kg/day） and prednisone（1 mg/kg/day）. Six controls with the same disease treated only with prednisone. Plasmal NO metabolites and the amount of total 24h urine protein around treatment were examined. Results The present results shows： the supernatant accumulation of NO metabolites was significantly decreased by CsA（0.1,1,10 μ mol/L）,expectly from 74.63 ±5.83 μ mol/L to 11.49 ± 1.72 μ mol/L, 9.78 ± 1.05μmol/L, 9.54 ± 0.83μmol/L.P〈0.01. In vivo,placmal NO metabolites of patients treated with CsA was decreased from, 197.58 ± 21.64μ mol/L to 125.39 ± 16.46 μ mol/L,P〈0.01;the amount of 24h urine protein from 4.53 ± 0.92g to 3.81 ± 1.26g,P〈0.01. The controls had no significant changes. Conclusion NO synthesis in response to TNF1^1A can be inhibited by CsA.

关 键 词：环胞霉素A 一氧化氮 膜性肾病 

分 类 号：R692[医药卫生—泌尿科学]