单纯疱疹病毒Ⅱ型gD糖蛋白核酸疫苗的构建及免疫观察  

The Construction and Preliminary Immunity of Herpes Simplex Virus Type 2 Glycoprotein D DNA Vaccine

作　　者：邵文爱[1] 李晓眠[2] 李梅[2] 

机构地区：[1]汕头大学医学院微生物与免疫学教研室,515031 [2]天津医科大学微生物教研室

出　　处：《天津医药》2006年第1期42-44,共3页Tianjin Medical Journal

摘　　要：目的:构建用于防治单纯疱疹病毒Ⅱ型(HSV-Ⅱ)引起的生殖器疱疹的DNA疫苗,并探讨其免疫保护作用。方法:利用PCR法从HSV-ⅡSav株中扩增出gD糖蛋白基因的全部编码序列,将其插入pcDNA3中,构建HSV-IIgD核酸疫苗,用PCR法、酶切法和测序法进行鉴定;将重组质粒转染COS-7细胞,用原位ELISA检测表达产物;对BALB/c小鼠进行肌肉免疫,用微量细胞中和试验、ELISA、脾T淋巴细胞增殖反应和病毒攻击试验检测免疫效果。结果:构建的HSV-ⅡgD核酸疫苗具有良好的免疫原性,对小鼠具有保护作用。结论:本实验成功构建出了具有免疫防御效应的HSV-ⅡgD核酸疫苗。Objective： To construct a DNA vaccine that can be used to prevent and treat the genital herpes which was leaded by HSV-2 and evaluate its immunization protection. Methods： The whole encoding sequence of the gD gene was amplified from HSV-2 Say strain by polymerase chain reaction then cloned into pcDNA3 to construct the HSV-2 gD nucleic acid vaccine which was confirmed strain by the PCR and restriction endonuclease analysis and sequence. The recombinant plasmid was transfected into the COS-7 cell. The expression products were analyzed by situ ELISA. The ELISA, microneutralization test, the spleen T lymphone proliferative responses and the virus challenge were employed to evaluate immune results which were received from muscular immunization to BALB/c mice. Results： The construction of the HSV-2 DNA nucleic acid vaccine had a good immunity and protect survival rates of mice. Conclusion： The immune HSV-2 nucleic acid vaccine is constructed successfully.

关 键 词：疱疹病毒2型 人 糖蛋白类 疫苗 DNA 小鼠 近交BALBC 

分 类 号：R373.11[医药卫生—病原生物学] R512.62[医药卫生—基础医学]