天癸更年软胶囊对老年雌性大鼠骨骼代谢影响的实验研究  

Experimental Study on Effect of Tiangui Gengnian Soft Capsule on Aged Female Rats with Osteoporosis

作　　者：刘波[1] 袁秉祥[1] 郭雄[2] 魏霞蓁[3] 赵丽梅[1] 康军[3] 陈东[3] 

机构地区：[1]西安交通大学医学院药理学系,西安710061 [2]西安交通大学教育部地方病,骨病研究所 [3]西安交通大学天奎医药生物公司

出　　处：《中国中西医结合杂志》2006年第2期135-139,共5页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家863专项基金(No.2003AA2Z3265)

摘　　要：目的观察天癸更年软胶囊对老年雌性大鼠血清雌激素及子宫、肝脏及骨骼等组织雌激素受体表达的影响,从受体及细胞因子方面探讨其抗老年骨质疏松的作用机理。方法青年组雌性大鼠、22月龄雌性大鼠(老年组)及天癸更年软胶囊低、中、高剂量组(0.72g/kg、1.80g/kg、4.50g/kg)、雌激素组(己烯雌酚0.02mg/kg)灌胃给药45天后,采用放免及酶免法测定大鼠血清雌激素E2、转化生长因子(TGFβ1)、胰岛素样生长因子(IGF1)水平;免疫组化SABC法分别检测骨、子宫、肝脏雌激素受体(ER)mRNA、TGFβ1以及IGF1的蛋白表达;地高辛探针原位杂交检测上述各组大鼠子宫、肝脏ERmRNA的表达。结果天癸更年软胶囊干预使大鼠血清E2、IGF1和TGFβ1增加,其中高剂量组TGFβ1值为(90.63±18.71)pg/L,与老年组比较,差异有显著性(P<0.01);大鼠子宫和肝脏ERmRNA表达及对肝脏TGFβ1、IGF1细胞因子蛋白表达均无明显影响(P>0.05)。结论天癸更年软胶囊改善绝经后骨骼代谢,缓解并纠正骨量丢失,其作用机理之一可能是通过雌激素受体途径及细胞因子(TGFβ1、IGF1)旁分泌/自分泌途径,促进成骨、抑制破骨来实现的。Objective To investigate the effect of Tiangui Gengnian soft capsule （TGC）, which mainly consists of seabuckthorn fatty acid, on serum estrogen and estrogen receptor （ER） in multiple target tissues as uterus, liver and bone, in aged female rats, in order to explore its mechanism from the aspects of receptor and cytokines. Methods Low （0.72 g/kg）, middle （1.80 g/kg） and high （4.50 g/kg） dose of TGC were admin istered by gastrogavage to young and aged （22 months old） female rats with osteoporosis for 45 days, and di ethylstilbestrol （0.02 mg/kg） was used as a positive control. The levels of serum estradiol （E2）, transforming growth factor β1 （TGF-β1 ）, insulin-like growth factor-1 （IGF-1） were measured by radioimmunoassay and ELISA method, the protein expression of their receptor in bone, uterus and liver was detected by SABC immunohistochemistry, and the mRNA expression of E2 in uterus and liver detected by in situ hybridization with digoxin probe. Results Intervention of TGC could cause increase of serum E2, IGF-1 and TGF-β1 levels, the TGF-β1 reached 90.63 ± 18.71 pg/L in the group administered with high dose, which was significantly different to that in the aged group （P 〈 0.01 ）. There was no obvious effect of the mRNA expression of E2 in uterus and liver, and no effect of TGF-β1 and IGF-1 in liver in rats. Conclusion TGC could improve the postmenopausal bone metabolism, alleviate and correct the bone loss, it is possibly realized by way of side-secretlng/auto-secreting of E2 receptor and cytokines （TGF-β1 and IGF-1 ） to improve the osteogenesis and inhibit the destruction of bone.

关 键 词：绝经后骨质疏松 天癸更年软胶囊 雌激素受体 细胞因子 

分 类 号：R285.5[医药卫生—中药学] R161[医药卫生—中医学]