脑热清对内毒素性发热家兔血管内皮细胞分泌功能的影响  

Effects of Naoreqing on Secretive Function of Vaso-endothelial Cells in Rabbits with Endotoxic Fever

作　　者：刘智勤[1] 蒋玉凤[1] 王秋华[1] 樊永平[2] 岳晓莉[1] 黄启福[1] 胡以明[2] 

机构地区：[1]北京中医药大学 [2]北京市天坛医院

出　　处：《中国中西医结合杂志》2006年第2期144-146,共3页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：北京市科委课题(No.954024200)

摘　　要：目的从血管内皮细胞分泌功能探讨脑热清(NRQ)口服液对内毒素性发热家兔的作用。方法复制内毒素性发热家兔模型,观察NRQ对家兔体温的影响,采用放免法测定血浆血栓素B2(TXB2)、6酮前列腺素F1α(6ketoPGF1α)以及内皮素(endothelin,ET)的含量,用发色底物法测定血浆组织纤溶酶原激活物(tPA)和组织纤溶酶原激活物抑制剂(PAI)的活性。结果NRQ+内毒素组的ΔT[(0.69±0.07)℃]、TRI2(4.85±0.57)、ET[(197.96±39.11)ng/L]和TXB2[(177.35±77.30)ng/L]的含量以及PAI[(0.84±0.01)AU/ml]的活性明显低于内毒素组的ΔT[(1.31±0.13)℃]、TRI2(8.44±0.98)、ET[(250.80±40.99)ng/L]和TXB2[(279.64±83.74)ng/L]的含量以及PAI[(0.86±0.01)AU/ml]的活性,两者比较差异有显著性(P<0.05,P<0.01);而6ketoPGF1α[(986.70±327.36)ng/L]的含量和tPA[(0.25±0.02)IU/ml]的活性明显高于内毒素组6ketoPGF1α[(507.81±170.01)ng/L]的含量和tPA[(0.21±0.02)IU/ml]的活性,两者比较差异亦有显著性(P<0.01)。结论NRQ可能通过改善发热家兔血管内皮细胞的分泌功能,起到扩张血管,加快体表散热,从而对内毒素性发热家兔的解热效应起到一定的整合作用。Objective To observe the antifebrile effect of Naoreqing （NRQ） oral liquid on secretive function of vaso-endothelial cells in rabbits with endotoxic fever. Methods Endotoxic fever rabbit model was duplicatedto observe the effects of NRQ on body temperature, blood levels of thromboxane B2 （TXB2）, 6-keto-prostaglandin F1α（6-keto PGF1α） and endothelin （ET） using radioimmunoassay, as well as activity of tissue-type plasminogen activator （t-PA） and plasminogen activator inhibitor （PAI） in plasma by chromophoric substrate assay. Results Comparisons of various indexes between the two groups showed significantly difference, i.e. the maximal increment of body temperature： 0.69 ± 0.07℃ vs 1.31 ± 0.13℃ （the NRQ treated group vs the untreated model group, the same hereafter） ; 2h thermal response index TRI2 4.85 ± 0.57 vs 8.44 ± 0.98; plasma ET content 197.96 ± 39.11 ng/L vs 250.80 ± 40.99 ng/L; TXB2 content 177.35 ± 77.30ng/L vs 279.64 ± 83.74 ng/L; activity of PAl 0.84 ± 0.01AU/ml vs 0.86 2 0.01 AU/ml; plasma 6-keto-PGF1α content 986.70 ± 327.36 ng/L vs 507.81 ± 170.01 ng/L; activity of t-PA 0.25 ± 0.02 IU/ml vs 0.21 ± 0.02 IU/ml （P 〈 0.05, P 〈 0.01）. Conclusion NRQ may improve secretive function of vaso-endothelial cells to dilate blood vessel and quicken heat dissipation through body surface, so as to play an integral antipyretic effect in rabbits with endotoxic fever.

关 键 词：发热 内毒素 内皮细胞 家兔 

分 类 号：R285[医药卫生—中药学]