DDA提高结核杆菌组合DNA疫苗免疫效果的研究  

DDA enhanced immnnogenicity and protection of combined DNA vaccine against Mycobacterium tuberculosis

作　　者：余大海[1] 田霞[1] 蔡宏[1] 朱玉贤[1] 

机构地区：[1]北京大学蛋白质工程及植物基因工程国家重点实验室,北京100871

出　　处：《高技术通讯》2006年第2期171-174,共4页Chinese High Technology Letters

基　　金：863计划（2002AA206411）资助项目.

摘　　要：将编码结核杆菌的三种抗原Ag85B，MPT64，MPT83的基因片段分别插入到真核表达载体中，混合后作为组合疫苗免疫小鼠，DDA作为佐剂提高了三价疫苗的免疫原性和免疫保护效果。添加DDA后Ag85B，MPT64，MPT83抗原特异的IFN-γ的含量分别为（265．37±79．2）U／ml，（185．31±58．3）U／ml，（108．13±54．4）U／ml，分别比非佐剂组高16U／ml，45U／ml，2U／ml。IL-4的含量在各组中相差不多，仅为纳克级。攻毒后细菌计数结果显示，肺脏和脾脏的载菌量在添加佐剂的三价组中降低了三个数量级，都达到了未加佐剂组相应脏器的一半菌量。病理切片显示结果与载菌量一致，添加佐剂组肺部淋巴细胞相对较少，巨噬细胞增多。因此，DDA作为佐剂提高了组合疫苗的免疫效果。A combined DNA vaccine encoding Ag85B, MPT64 and MPT83 of Mycobacterium tuberculosis was formulated into DDA to immunize mice and to evaluate the immunogenicity and protective efficacy of the vaccine in the immunized mice. The combined vaccine formulated in DDA induced a much enhanced Th1-type cellular response with higher amounts of IFN-γ being produced compared to the combined DNA vaccine. In this group, antigens specific IFN-γ for Ag85B, MPT64, MPT83 were （265.37 ± 79.2）U/ml, （ 185.31 ± 58.3） U/ml, （ 108.13 ± 54.4） U/ml, respectively, which were 16 U/ml,45 U/ml and 2 U/ml higher than that of the combined DNA vaccine group without DDA. The bacterial CFU was reduced half in lungs and in spleens relative to the same cembined vaccine without DDA. The lungs of this group of mice showed less damage due to influx of epithelioid macrophages and less neutrophils. DDA promoted the iraname response of combined DNA vaccine in mice.

关 键 词：结核分支杆菌 组合疫苗 佐剂DDA 免疫效果 

分 类 号：S942.5[农业科学—水产养殖] S858.31[农业科学—水产科学]