荧光定量PCR检测HBV DNA及其与Pre-S1和HBeAg关系探讨  

The analysis of the relationship between the HBV serological markers and the HBV DNA detected by FQ-PCR

作　　者：管世鹤[1] 方有兵[1] 黄争艳[1] 陆应玉[1] 刘华平[1] 

机构地区：[1]安徽医科大学第一附属医院检验科,合肥230022

出　　处：《安徽医学》2006年第2期94-96,共3页Anhui Medical Journal

摘　　要：目的探讨慢性乙肝患者HBVDNA与血清标志物前S1抗原(Pre-S1)和HBeAg的相互关系。方法采用荧光定量聚合酶链反应技术(FluorescencequantitativePCR,FQ-PCR)和酶联免疫吸附试验(ELISA)对931份HBV-M不同阳性模式及35份HBV-M全阴模式血清进行HBVDNA及其标志物检测。结果在931例不同阳性模式乙肝患者中,HBeAg阳性总检出率为20.7%,Pre-S1抗原阳性总检出率为30.2%,HBVDNA阳性总检出率为45.3%。在422例HBVDNA(+)乙肝患者中,Pre-S1抗原阳性检出率为57.3%,HBeAg阳性检出率为44.1%。在281例Pre-S1(+)乙肝患者中,HBVDNA阳性符合率达86.1%,在Pre-S1(-)组HBVDNA阳性率仅为27.7%。结论HBVDNA检测是反映HBV复制最直接的分子生物学指标;Pre-S1抗原和HBeAg是反映HBV复制的血清学指标,Pre-S1抗原甚至比HBeAg更灵敏地反映HBV复制。Objective To investigate the relationship between the HBV serological markers and the HBV DNA in the chronically hepatitis B virus infected patients. Methods The ELISA was used to analyze these serum markers of HBV in 931 case chronic hepatitis B patients who were included in this study. The markers,like as HBsAg, anti - HBs, HBeAg, anti - HBe, anti -HBc and Pre -S1 was determined by ELISA, while the HBV DNA was determined by fluorescence quantitative PCR（ FQ - PCR） assay. The relationship of Pre -S1 antigen, HBeAg and HBV DNA involved in the HBV replication was also analyzed simultaneously. Results The total positive rate of HBeAg in these patients just was 20.7% , while the total positive rate of Pre - S1 antigen in these patients was 30.2% ,and the positive rate of HBV DNA reached 45.3%. We found that the total positive rate of Pre - S1 antigen was 57.3% while the total positive rate of HBeAg was just 44.1% in 422 case of HBV DNA positive patients. Meanwhile,we found that there was a significant difference of the positive rate of HBV DNA between Pre -S1 （ ＋ ） and Pre-S1 （ - ）groups （ P 〈 0.01 ）. Conclusion The HBV DNA determined by FQ - PCR is the most directly molecular biology marker, which reflects the HBV replication. Both Pro - S1 antigen and HBeAg are the more important serological markers of HBV replication. Pre - S1 is even more sensitive than HBeAg to reflect the HBV replication in chronic hepatitis B patients.

关 键 词：荧光定量PCR 酶联免疫吸附试验(ELISA) HBV DNA HBV标志物 

分 类 号：R450[医药卫生—治疗学]