^3H-thymidine渗入法测定Urocortin对大鼠血管平滑肌细胞增殖的影响  

THE EFFECT OF UROCORTIN ON PROLIFERATION OF RAT VASCULAR SMOOTH MUSCLE CELLS MEASURED BY ^3H-thymidine

作　　者：罗惠媛[1] 周国栋 陶金[3] 陈洁[3] 

机构地区：[1]江苏省扬州卫生学校临床教研组,江苏扬州225007 [2]内蒙古通辽市红十字会中心血站,内蒙古通辽028041 [3]南京医科大学药理系,江苏南京210029

出　　处：《实用临床医药杂志》2006年第1期37-38,共2页Journal of Clinical Medicine in Practice

基　　金：江苏省卫生厅教育研究室科研项目(J200410)

摘　　要：目的探讨Urocortin(UCN)对大鼠血管平滑肌细胞增殖的影响。方法分离、培养大鼠血管平滑肌细胞,分别加入不同浓度的UCN以及UCN+Astressin,UCN+Glybenclamide培养,采用3H-thymidine掺入法,观察不同浓度UCN对血管平滑肌细胞增殖的影响,及其相关作用机制。结果随着Urocortin浓度的增高,VSMC对3H-thymidine摄取率呈下降趋势,而该作用不受Astressin或Glybenclamide的影响。结论Urocortin具有抑制大鼠血管平滑肌细胞增殖的作用,其作用不是通过CRF受体或KATP通道。Objective To investigate the effect of urocortin（UCN） on the proliferation of rat vascular smooth muscle cells（VSMC）. Methods Rat aortic VSMCs were isolated from rats＇ thoracic aorta. VSMCs treated with astressin, Glybenclamide and different concentrations of UCN. The effect of UCN on the proliferation of VSMC was detected by using the ^3H-thymidine incorporation assay. Results With UCN（10^-10 to 10^-5 mol/L） condensing, UCN ingesting CSMC decreased, which was not affected by astressin or Gylbenclamide. Conclusion UCN inhibits the proliferation of rat vascular smooth muscle cells. Forthmore, this effect was not via CRH receptor or K -ATP channels.

关 键 词：Umeortin ^3H-thymidine 血管平滑肌细胞(VSMC) 增殖 

分 类 号：R544.1[医药卫生—心血管疾病]