估算供体-受体对荧光共振能量传递效率的研究  

Investigation of Calculating the FRET Efficiency of Donor-Acceptor Pair

作　　者：王进[1] 王晶[1] 孙依红[1] 孟继武[1] 

机构地区：[1]中国海洋大学光学光电子实验室,山东青岛266071

出　　处：《光谱学与光谱分析》2006年第2期279-281,共3页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金(10074012);青岛市科技局(02-2-kj-yj-26)和(04-3-GX-33)资助项目

摘　　要：在有机白光LED的研究中,能量传递效率计算方法的研究是一项很有意义的工作。作者在总结前人工作的基础上,提出了一种利用有机分子发射谱和激发谱估算有机分子荧光共振能量传递(FRET)效率相对值的新方法,这种方法的优点是数据处理过程简单,对实验仪器设备要求不高,并且不需要通常计算方法所需的荧光量子产率等参数,缺点是只能计算能量传递的相对值。利用这种方法计算了甲萘酚、溴甲酚紫、萤光素钠盐三种能量受体和能量供体核黄素在不同浓度下能量传递效率的相对值,计算结果与实验结果基本符合,验证了此方法的合理性。The research on the method of calculating FRET efficiency is important to organic white LED research. In the present article, a new method of calculating the FRET efficiency of donor-acceptor pair by means of emission and excitation spectra was investigated. This method is easy to use to process data, needs only normal experiment equipments and doesn＇t need the parameters such as fluorescence quantum yield. However it can only be used to calculate comparative result. With this method, the FRET efficiency of fluorescein sodium, α-naphthol, bromocresol purple and riboflavin with different concentrations was studied, and the results match the experimental results completely, so this method has proved to be reasonable.

关 键 词：供体发射谱 受体激发谱 荧光共振能量传递效率 乘积积分估算法 

分 类 号：O433.5[机械工程—光学工程]