槲皮素的共振光散射光谱研究  

Resonance Light Scattering of Quercetin

作　　者：曾铭[1] 李树伟[1] 邓嘉利[1] 李小燕[1] 赵可清[1] 

机构地区：[1]四川师范大学化学学院,四川成都610066

出　　处：《光谱学与光谱分析》2006年第2期317-320,共4页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金(50473062)资助项目

摘　　要：研究了槲皮素（Qu）的共振光散射光谱和吸收光谱，结果表明，pH在3．30～6．50范围内，Qu的共振光散射信号很强且稳定，当pHi6．50时，共振光散射强度随pH的增大而迅速减小。在pH4．00的B-R缓冲溶液中，在λ=497nM处，共振光散射强度在一定浓度范围内与Qu的浓度成线性关系，线性范围为0．0～3．0×10^-4 mol·L^-1，相关系数r〉0．999，检测限为3．1×10^-7 mol·L^-1。同时运用量子化学计算方法对Qu分子内、分子间氢键进行了计算，理论计算表明：Qu共振光信号增强的原因是Qu分子通过4—4’分子间氢键聚合形成了超分子聚合体，这一结论和实验得到的光谱数据完全吻合。Resonance light scattering（RLS） and absorption spectra of Quercetin were studied in the present paper. In the solution with pH 3. 30 to pH 6.50, the RLS singal was very strong and stable, but decreased sharply with a increase in pH when pH 〉6.50. In the B-R buffer solution at pH 4.00 , the RLS intensity at 497 nm was maximum and proportional to the concentration of quercetin. The linear range is 0. 0-3.0 × 10^- 4 mol · L^-1 with the detection limit of 3. 1 × 10^-7 mol · L^-1. Quantum chemistry calculation showed that this enhancement of RLS occurred because the neutral molecules of quercetin assembled into supermolecular aggregates by 4-4’hydrogen-bond. This result completely accords with the spectral data from experiments.

关 键 词：共振光散射 槲皮素 超分子 氢键 

分 类 号：O657.3[理学—分析化学]