中药复方“芪丹煎”对家兔碱性成纤维细胞生长因子的影响  

Effect of Decoction Qidan Liquid on Basic Fibroblast Growth Factor in Artherosclerotic Rabbits

作　　者：焦玲霞[1] 周俊琴[2] 黄东华[2] 

机构地区：[1]河北医科大学图书馆,河北省石家庄市050017 [2]河北医科大学中医学院,河北省石家庄市050091

出　　处：《中国动脉硬化杂志》2005年第5期560-562,共3页Chinese Journal of Arteriosclerosis

基　　金：河北省科技攻关项目(02276104D-20)

摘　　要：目的观察中药复方'芪丹煎'对实验性动脉粥样硬化家兔腹主动脉碱性成纤维细胞生长因子的影响.方法将24只4月龄健康雄性新西兰白兔 (体重2.4～2.9 kg),随机均分为四组,即正常组、模型组、芪丹煎组和硝苯地平组.模型组喂饲高脂颗粒饲料,芪丹煎组每兔每天喂饲按100 g饲料含生药60 g的量制成的高脂颗粒饲料,硝苯地平组每兔每天喂饲按40 mg硝苯地平的量制成的高脂颗粒饲料,正常组喂饲普通颗粒饲料.12周后放血处死家兔,取胸主动脉用于HE染色,腹主动脉用于碱性成纤维细胞生长因子的免疫组织化学检测.结果与模型组比较,芪丹煎组碱性成纤维细胞生长因子的表达量明显减少(P<0.01);与硝苯地平组比较,芪丹煎组碱性成纤维细胞生长因子的表达量也有明显差异(P<0.05).病理检测结果发现芪丹煎组胸主动脉病变程度较模型组明显减轻.结论对腹主动脉碱性成纤维细胞生长因子的抑制作用可能是芪丹煎抗动脉粥样硬化的机制之一.Aim To observe the effeet of Qidan liquid on the basie fibroblast growth faetor （bFGF） of abdominal aorta in experimental artherosclerotie rabbits. Methods Twenty-four white, four months old, male, New Zealand rabbits whose weights were from 2.4 kg to 2.9 kg were divided into four groups at random： normal group, model group, Qidan hquid group and nifedipine group. Rabbits of normal group were fed with common rabbit forage, the rabbits of Qidan liquid group were fed with Qidanliquid（3kg/L）. A rabbit was fed 20 mL everyday. The rabbits of nifedipine group were fed with nifedipine （ 40 mg everyday to each rabbit）. After twelve weeks, after the rabbits were put to death by exsanguinate, thoracic aorta and abdomial aorta were took out and kept in 10% neutralizing formaldehyde stabilization liquid, for the impection of HE staining and for the detection of bFGF by immunohistochemistry. Results Compared with model group , the expression of bFGF in Qidan liquid group was decreased remarkably （ P 〈 0.01 ）. Compared with nifdepine group, the expression of bFGF was decreased sharply in Qidan liquid group （P〈0.05）. Compared withmedelgroup, the degree of atheroselerofie lesion in aorta was redueed in Qidan liquid group. Conclusion Inhibiting the expression of bFGF in abdominal aorta is probably the mechanism of Qidan liquid in antiartheroselerosis.

关 键 词：中药学 中药抗动脉粥样硬化的作用 免疫组织化学 芪丹煎 碱性成纤维细胞生长因子 动脉粥样硬化 

分 类 号：R28[医药卫生—中药学]