骨碎补类黄酮对氯化汞所致的急性肾衰竭大鼠模型的保护作用  

Protective Effects of Flavonoid of Drynaria Fortunei in ARF Rats with Mercuric Chloride

作　　者：蒋文功[1] 出蒲照国 方敬爱[3] 井上嘉彦[3] 

机构地区：[1]广东药学院临床医学院,广州510224 [2]日本昭和大学藤が丘病院肾内科 [3]山西医科大学第一医院肾病科,太原030001

出　　处：《中国中西医结合肾病杂志》2006年第2期75-79,i0001,共6页Chinese Journal of Integrated Traditional and Western Nephrology

摘　　要：目的:探讨骨碎补类黄酮提取物(flavonoid fraction,FF)对氯化汞(HgCI2)所致的急性肾衰竭(ARF)大鼠模型的保护作用。方法:利用HgCI2诱导的ARF动物模型,将大鼠随机分成正常对照组、HgCI2(模型组)、HgCI2+FF组、FF组。在实验第24、48、72h检测血生化指标,光镜观察肾组织改变,免疫组化染色检测增殖细胞核抗原(PCNA)、ED-1在肾组织的表达,同时检测肾组织MDA及GSH含量。结果:HgCI2+FF组肾脏病理改变比HgCI2组减轻,同时血肌酐下降(P<0.05);免疫组化显示PCNA、ED-1在肾间质表达下调,均与HgCI2组有统计学差异(分别P<0.01及P<0.05);FF还可预防肾组织因HgCI2毒性所致的MDA含量升高,GSH下降(P<0.05)。结论:FF通过抗氧化作用,减轻HgCI2诱导的急性肾衰竭。Objective：We investigate the effects of the flavonoid fraction（FF） from Drynaria fortunei on the rats with acute renal failure （ARF） caused by mercuric chloride（HgCl2）. Methods：By means of the animal model of ARF induced by HgCl2, BALB/c rats were divided into 4 groups：normal group,model group（HgCl2） ,HgCl2 ＋ FF group and FF group. At the 24th ,48th and 72th hours after the insult, serum creatinine（Scr） , renal pathological changes, PCNA and ED- 1 cells in renal tissues were studied, we also determined the renal content of malondialdehyde（MDA） and glutathione（GSH）. Results：Pathological change in HgCl2 ＋ FF group was more slight than that in HgCl2 group, meanwhile, the Scr was reduced in FF treated group （P〈0.05） ;The PCNA and ED- 1 positive cells decreased in the HgCl2 ＋ FF group （P〈0.01 and P〈0.05,respectively） ; Increrment in renal content of MDA and decrease in GSH resulting from HgCI2 toxicity were prevented by FF treatment（ P 〈 0.05）. Conclusion： FF treatment might ameliorate HgCl2 - induced ARF through its antioxidant function.

关 键 词：急性肾衰竭 骨碎补 氧化应激 氯化汞 

分 类 号：R285.5[医药卫生—中药学]