早期生长反应基因-1在自体移植静脉中的表达研究  

The expression and significance of early growth response gene-1 in autogenous vein graft in rats

作　　者：刘程伟[1] 杨军[1] 张雪松[2] 杨德华[1] 张强[1] 胡新华[1] 

机构地区：[1]中国医科大学附属第一医院血管外科,沈阳110001 [2]佳木斯大学附属第一医院放射科

出　　处：《中华普通外科杂志》2006年第2期98-101,共4页Chinese Journal of General Surgery

基　　金：国家自然科学基金资助项目(30400435);宁省教育厅科研基金资助项目(202013161);博士启动基金资助项目(20041053)

摘　　要：目的研究自体静脉移植后早期生长反应基因-1(Egr-1)表达的动态变化,探讨其在内膜增生(IH)中的作用。方法 Wistar大鼠90只,建立自体静脉移植模型。术后分为1、2、6、24 h,3、 7、14、28、42 d组,取正常静脉作为对照。应用原位杂交和RT-PCR方法检测Egr-1 mRNA的表达,联合应用Western blot蛋白印迹和免疫组织化学方法检测Egr-1的蛋白表达。结果自体静脉移植后, Egr-1 mRNA和Egr-1蛋白的表达具有双相变化。移植后1 h,Egr-1 mRNA基因表达值为2．21±0．85。 6-24 h时下降,7 d时重新升高,28 d达高峰为3．16±1．14。移植早期2 h:Egr-1蛋白的表达阳性率为 31％±6％,24 h至3 d下降,28 d达高峰为41％±8％。免疫组化显示:移植早期Egr-1蛋白阳性表达主要位于中膜血管平滑肌细胞(VSMCs)和单核细胞／巨噬细胞;移植后期Egr-1蛋白阳性表达主要位于新生内膜和中膜的VSMCs,同时部分内皮细胞也有表达。结论移植静脉内膜增生与Egr-1的激活及表达关系密切,因而与移植静脉内膜增生及狭窄有关。Objective To investigate the expression of early growth response gene-1 in autogenous vein graft in rats, and its role in intimal hyperplasia （ IH ）. Methods Autogenous vein graft model was established in 90 Wistar rats. Grafts were harvested at 1,2,6,24 hours,and 3,7,14,28,42 days after surgery. Normal veins serve as control. Egr-1 mRNA was measured by RT-PCR and in situ hybridization. Western blotting and immunohistochemistry were used to detect the protein expression of Egr-1. Results The expression of Egr-1 mRNA and Egr-1 protein experienced biphasic changes. At 1 hour, the value of gene expression of Egr-1 mRNA was 2. 21 ±0. 85, declining at 6 and 24 hours, and upgrading at 7 days, culminating at 3.16 ± 1.14 at 28 days. Egr-1 protein expression was detected at 2 hours, with the positive expression rate of 31% ±6%. The level of Egr-1 protein was decreased from 24 hours to 3 days. A peak was reached at 41% ± 8% at 28 days. Immunoreative Egr-1 was located within vascular smooth muscle cells （VSMCs） and monocytes/macrophages in the media at the early phase of day 7 and 14, and in neointimal and medial VSMCs at later phase of day 28. Egr-1 was also present in the endolumial endothelial cells. Conclusion In autogenous vein graft, Egr-1 plays an important role in the proliferation of VSMCs in autogenous vein graft.

关 键 词：移植 自体 静脉 早期生长反应基因-1 

分 类 号：R654.3[医药卫生—外科学]